Establishment and comparison of two methods to produce a rat model of mammary gland hyperplasia with hyperprolactinemia
Título del documento: Establishment and comparison of two methods to produce a rat model of mammary gland hyperplasia with hyperprolactinemia
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000452172
ISSN: 1984-8250
Autores: 1
2
1
1
1
1
1
Instituciones: 1The Third Hospital of Wu-han, Wuhan, Hubei. China
2Shihezi University, Medical School, Shihezi, Xinjiang. China
Año:
Volumen: 58
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés This study aimed to establish and compare models of mammary gland hyperplasia (MGH) with hyperprolactinemia (HPRL) using two different methods. The models provide information on the relationship between mammary gland hyperplasia and associated hormones. Model A was constructed using intramuscular injections of estradiol benzoate injection (EBI), followed by progesterone (P), and then metoclopramide dihydrochloride (MDI). Model B was designed by administering MDI, follow by EBI, and then P intramuscularly. Model B showed higher MGH progression compared with model A. Notably, increase in estradiol (E2) was negatively correlated with prolactin (PRL) secretion. However, PRL levels in model B were significantly higher compared with the levels in model A. Estrogen (ER), prolactin receptor (PRLR), and progesterone receptor (PR) mRNA and protein expression levels in model B rats were positively correlated with changes in the corresponding hormone levels. However, E2, P, and PRL levels in model A showed no direct relationship with levels of the mRNAs of related hormones and protein expression levels. Our results suggest that model B is an appropriate model of MGH with HPRL that can be used to perform further studies about the interactions of the E2, P, and PRL hormones in this disorder
Disciplinas: Medicina
Palabras clave: Endocrinología,
Farmacología,
Medicina experimental,
Estradiol,
Progesterona,
Prolactina,
Hiperplasia mamaria,
Hiperprolactinemia
Keyword: Endocrinology,
Pharmacology,
Experimental medicine,
Estradiol,
Progesterone,
Prolactin,
Mammary hyperplasia,
Hyperprolactinemia
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