| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451810 |
| ISSN: | 1984-8250 |
| Autores: | Tian, Haitao1 Zhao, Pan1 Li, Jing1 Deng, Zhipeng1 Pu, Gaobin1 Wang, Hong2 |
| Instituciones: | 1Shandong University of Traditional Chinese Medicine, School of Pharmaceutical Sciences, Jinan, Shandong. China 2Jiaozhou People’s Hospital, Qingdao, Shandong. China |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | A simple and selective liquid chromatography tandem with mass spectrometry (LC-MS/ MS) method for quantification of lobetyolin in rat plasma was developed and validated. Chromatographic separation was achieved on a Thermo ODS C18 reversed-phase column using 0.1% aqueous formic acid-methanol (50:50, v/v) in an isocratic elution mode at a flow rate of 0.4 mL.min-1. LC/MS performance was done in a positive ion ESI mode and the MS/MS transitions were monitored at m/z 419.3 [M+Na]+ → m/z 203.1 for lobetyolin and m/z 394.9 [M+Na]+ → m/z 231.9 for IS, respectively. The assay exhibited a linear dynamic range over 1.0-500 ng.mL-1 for lobetyolin in plasma. Both the precision (%RSD) and accuracy (RE%) were within acceptable criteria (<15%). Recoveries ranged from 87.0% to 95.6%, and the matrix effects were from 91.0% to 101.3%. After oral administration, the peak plasma concentration of lobetyolin was obtained as 60.1 ng.mL-1 at 1.0 h. The proposed LC-MS/MS method could be applied to a pharmacokinetic study employing 66 samples from 6 Wistar rats |
| Disciplinas: | Química |
| Palabras clave: | Química farmacéutica, Lobetiolina, Farmacocinética |
| Keyword: | Medicinal chemistry, Lobetyolin, Pharmacokinetics |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
