Determination of ATP and its metabolites in dietary energy supplements by capillary electrophoresis



Título del documento: Determination of ATP and its metabolites in dietary energy supplements by capillary electrophoresis
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000452211
ISSN: 1984-8250
Autores: 1
Instituciones: 1Inonu University, Faculty of Pharmacy, Malatya. Turquía
Año:
Volumen: 58
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés This study aimed to develop a simple and fast capillary electrophoresis (CE) method for the simultaneous determination of adenosine triphosphate (ATP) and its metabolites in dietary energy supplements. Reverse polarity separation mode for faster separation of the three strong negatively charged analytes and capillaries with a 25 µm inner diameter was employed. At -433 V/cm field strength at background electrolyte (BGE) consist with 0.1 M tris-HCl, 0.5 mM tetradecyltrimethylammonium chloride (TTAC) as positively charged surfactant and 0.3 mg/mL hydroxypropylmethylcellulose (HPMC) to reduce the electroosmotic flow (EOF), a complete separation of the three species were achieved in less than 15 minutes. The data acquisition was conducted at a wavelength of 254 nm. Three different commercialised dietary energy supplements were analysed
Disciplinas: Química
Palabras clave: Química analítica,
Química de alimentos,
Suplementos alimenticios,
Contenido energético,
ATP,
Electroforesis por capilaridad
Keyword: Analytical chemistry,
Food chemistry,
Food supplement,
Energy content,
ATP,
Capillary electrophoresis
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