| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451251 |
| ISSN: | 1984-8250 |
| Autores: | Amparo, Tatiane Roquete1 Rodrigues, Ivanildes Vasconcelos2 Seibert, Janaína Brandão3 Almeida, Tamires Cunha1 Cabral, Vivette Appolinário Rodrigues4 Vieira, Paula Melo de Abreu5 Brandao, Geraldo Célio1 Oliveira, Mauro Lúcio Gonçalves de6 Silva, Glenda Nicioli da1 Santos, Orlando David Henrique dos1 Vieira, Sidney Augusto1 Teixeira, Luiz Fernando Medeiros1 Souza, Gustavo Henrique Bianco de1 |
| Instituciones: | 1Universidade Federal de Ouro Preto, Escola de Farmacia, Ouro Preto, Minas Gerais. Brasil 2Universidade Federal de Juiz de Fora, Departamento de Farmacia, Governador Valadares, Minas Gerais. Brasil 3Universidade Federal de Ouro Preto, Nucleo de Pesquisas em Ciencias Biologicas, Ouro Preto, Minas Gerais. Brasil 4Universidade Federal de Lavras, Departamento de Ciencias Florestais, Lavras, Minas Gerais. Brasil 5Universidade Federal de Ouro Preto, Departamento de Ciencias Biologicas, Ouro Preto, Minas Gerais. Brasil 6Instituto Mineiro de Agropecuaria, Belo Horizonte, Minas Gerais. Brasil |
| Año: | 2020 |
| Volumen: | 56 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Due to the increase of bacterial resistance, the search for new antibiotics is necessary and the medicinal plants represent its most important source. The aim of this study was to evaluate the antibacterial property of extract and fractions from Protium spruceanum leaves, against pathogenic bacteria. By means of diffusion and microdilution assays, the crude extract was active against the nine bacteria tested being the hydromethanolic fraction the most active. During phytochemical procedures, procyanidin (1) and catechin (2) were identified as the main antibacterial constituents of this fraction. In silico results obtained using PASSonline tool indicated 1 and 2 as having good potential to interact with different targets of currently used antibiotics. These results no indicated potential to none DNA effect and indicated the cell wall as mainly target. Electrophoresis result supported that had no DNA damage. Cell wall damage was confirmed by propidium iodide test that showed increased membrane permeability and by cell surface deformations observed in scanning electronic microscopy. The in vitro assays together with the in silico prediction results establish the potential of P. spruceanum as source of antibacterial compounds that acts on important bacterial targets. These results contribute to the development of natural substances against pathogenic bacteria and to discovery of new antibiotics |
| Disciplinas: | Química, Biología |
| Palabras clave: | Fitoquímica, Angiospermas, Catequina, Procianidinas, Actividad antibacteriana, Protium spruceanum, Burseraceae |
| Keyword: | Phytochemistry, Angiosperms, Catechin, Procyanidins, Antibacterial activity, Protium spruceanum, Burseraceae |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
