5-Aminolevulinic acid photoactivated over planktonic and biofilm forms of Enterococcus faecalis as a pharmacological therapy alternative
Título del documento: 5-Aminolevulinic acid photoactivated over planktonic and biofilm forms of Enterococcus faecalis as a pharmacological therapy alternative
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451337
ISSN: 1984-8250
Autores: 1
2
2
1
3
3
Instituciones: 1Universidad Autónoma de Sinaloa, Facultad de Odontología, Culiacán, Sinaloa. México
2Universidad Autónoma de San Luis Potosí, Programa de Doctorado en Ciencia e Ingeniería de Materiales, San Luis Potosí. México
3Universidad Autónoma de San Luis Potosí, Facultad de Odontología, San Luis Potosí. México
Año:
Volumen: 56
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés The purpose of the study was to evaluate the antibacterial effect of protoporphyrin IX (PpIX) generated by the exogenous administration of 5-aminolevulinic acid or δ-ALA and activated with an argon laser over a planktonic and biofilm of Enterococcus faecalis (E. faecalis) as a pharmacological therapy alternative. A planktonic strain of E. faecalis was cultured with a solution of ∂-ALA (40 µg/mL)-thioglycolate solution for 13 min, and a biofilm of E. faecalis was cultured in a δ-ALA (80 µg/mL)-thioglycolate solution for 13 min. Then, both were irradiated with an argon laser. Finally, the antibacterial effect was evaluated by counting the CFU in planktonic form, and a LIVE/DEAD viability cell test. The production and accumulation of PpIX from exogenously administered δ-ALA on E. faecalis in planktonic and biofilm forms was confirmed by spectrofluorometry. The irradiation of PpIX with an argon laser produced an antibacterial effect on E. faecalis in planktonic and biofilm form, even without biofilm disruption, at a concentration of 40 µg/mL and 80 µg/mL of δ-ALA, respectively. The exogenous administration of δ-ALA in combination with laser irradiation on planktonic and biofilm forms of E. faecalis produces an effective antibacterial effect as complement or alternative to pharmacological therapies
Disciplinas: Medicina
Palabras clave: Odontología,
Farmacología,
Terapia fotodinámica,
Biopelículas,
Acido 5-aminolevulínico,
Fotoactivación,
Protoporfirinas,
Actividad antibacteriana,
Enterococcus faecalis
Keyword: Dentistry,
Pharmacology,
Photodynamic therapy,
Biofilms,
5-aminolevulinic acid,
Photoactivation,
Protoporphyrins,
Antibacterial activity,
Enterococcus faecalis
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