| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000308993 |
| ISSN: | 0104-6632 |
| Autores: | Azzoni, A.R1 Takahashi, K Woodard, S.L2 Miranda, E.A Nikolov, Z.L |
| Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Engenharia Quimica, Campinas, Sao Paulo. Brasil 2ProdiGene, College Station, Texas. Estados Unidos de América |
| Año: | 2005 |
| Periodo: | Sep |
| Volumen: | 22 |
| Número: | 3 |
| Paginación: | 323-330 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Protein expression in transgenic plants is considered one of the most promising approaches for producing pharmaceutical proteins. As has happened with other recombinant protein production schemes, the downstream processing (dsp) of these proteins produced in plants is key to the technical and economic success of large-scale applications. Since dsp of proteins produced transgenically in plants has not been extensively studied, it is necessary to broaden the investigation in this field in order to more precisely evaluate the commercial feasibility of this route of expression. In this work, we studied the substitution of an IMAC chromatographic step, described in previous work (Azzoni et al., 2002), with ion-exchange chromatography on SP Sepharose Fast Flow resin as the second step in the purification of recombinant aprotinin from transgenic maize seed. The main goal of this second purification step is to separate the recombinant aprotinin from the native corn trypsin inhibitor. Analysis of the adsorption isotherms determined at 25°C under different conditions allowed selection of 0.020 M Tris pH 8.5 as the adsorption buffer. The cation-exchange chromatographic process produced a high-purity aprotinin that was more than ten times more concentrated than that generated using an IMAC step |
| Disciplinas: | Química |
| Palabras clave: | Ingeniería química, Biotecnología, Proteínas recombinantes, Maíz transgénico, Inhibidores de proteasas, Aprotinina, Purificación, Cromatografía de intercambio iónico |
| Keyword: | Chemistry, Chemical engineering, Biotechnology, Recombinant proteins, Transgenic corn, Protease inhibitors, Aprotinin, Purification, Ion exchange chromatography |
| Texto completo: | Texto completo (Ver HTML) |
