| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000308746 |
| ISSN: | 0104-6632 |
| Autores: | Cherry, J1 Ko, S Grainger, R Prokop, A Tanner, R. D |
| Instituciones: | 1Vanderbilt University, Department of Chemical Engineering, Nashville, Tennessee. Estados Unidos de América |
| Año: | 2000 |
| Periodo: | Jun |
| Volumen: | 17 |
| Número: | 2 |
| Paginación: | 233-238 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | There are many methods for separating and purifying proteins from dilute solutions, such as salting out/precipitation, adsorption/chromatography, foam fractionation, and droplet fractionation. In order to determine the optimal condition for a selected separation and purification process, an objective function is developed. The objective function consists of three parameters, which are the protein mass recovery, the separation ratio, and the enzymatic activity ratio. In this paper the objective function is determined as a function of the pH of the bulk solution for egg albumin, cellulase, and sporamin (for foam fractionation) and invertase ( for droplet fractionation). It is found that the optimal pH for all the systems except for cellulase is near their isoelectric point |
| Disciplinas: | Química |
| Palabras clave: | Ingeniería química, Separación de proteínas, Proceso de fraccionamiento de espuma, Proceso de fraccionamiento por goteo, Compensaciones |
| Keyword: | Chemistry, Chemical engineering, Protein separation, Foam fractionation process, Droplet fractionation process, Tradeoffs |
| Texto completo: | Texto completo (Ver HTML) |
