| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000308998 |
| ISSN: | 0104-6632 |
| Autores: | Makino, Y1 Lima, P.S.C Filho, F.M Rodrigues, M.I |
| Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos, Campinas, Sao Paulo. Brasil |
| Año: | 2005 |
| Periodo: | Dic |
| Volumen: | 22 |
| Número: | 4 |
| Paginación: | 539-545 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The enzyme inulinase is used to produce oligosaccharides and fructose, with up to 95% fructose in a single stage of inulina hydrolysis. With in the aim to purify the enzyme, studies on the conditions of enzyme adsorption in an expanded-bed column were conducted using phosphate and tris-HCl buffers. The inulinase used in this work was obtained from Kluyveromyces marxianus NRRL Y-7571 by fermentation in an industrial medium. Using the anionic resin Streamline DEAE, the adsorption equilibrium time was determined. It was observed that the adsorption isotherm follows the Langmuir model; the parameters for the maximum amount of adsorbed inulinase (Qm) and the dissociation constant (k d) were determined. With 0.05 M sodium phosphate buffer at pH 6.0, the parameter values 1428 UI/mL and 2 UI/mL with a correlation coefficient of 0.96 were obtained. For 0.02 M tris-HCl buffer at pH 7.5, the same parameters were 5000 UI/mL and 0.05 UI/mL with a correlation coefficient of 0.99. The best purification conditions for the fixed bed were shown to be a 0.4 M phosphate buffer with NaCl as eluter, a purification factor of 11.4, and a recovery yield of up to 79% |
| Disciplinas: | Química |
| Palabras clave: | Ingeniería química, Enzimas, Adsorción, Resinas aniónicas, Inulinasa, Kluyveromyces marxianus |
| Keyword: | Chemistry, Chemical engineering, Enzymes, Adsorption, Anionic resins, Inulinase, Kluyveromyces marxianus |
| Texto completo: | Texto completo (Ver HTML) |
