| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000309009 |
| ISSN: | 0104-6632 |
| Autores: | Nucci, E.R1 Silva, R.G Gomes, T.C Giordano, R.C Cruz, A.J.G |
| Instituciones: | 1Universidade Federal de Sao Carlos, Departamento de Engenharia Quimica, Sao Carlos, Sao Paulo. Brasil |
| Año: | 2005 |
| Periodo: | Dic |
| Volumen: | 22 |
| Número: | 4 |
| Paginación: | 521-527 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Penicillin G acylase (PGA) is an important enzyme used as biocatalyst in the production of semisynthetic beta-lactam antibiotics. Many microorganisms produce this enzyme and recombinant Escherichia coli has been preferred use for industrial applications. Bacillus megaterium is one of the microorganisms that excretes this enzyme into the medium. As a consequence, separation and purification steps are simplified. On-line measurement of enzyme activity during cultivation using in-situ sensors is a difficult task in the industrial environment due to the lack of robust and inexpensive instrumentation. This work presents the results of a fuzzy logic algorithm used to determine the moment of maximum enzyme concentration during Bacillus megaterium cultivations in an aerated and stirred, automated lab-scale bioreactor. The fuzzy algorithm was written in Fortran, compiled as a dynamic link library and implemented on a platform developed in MS-Visual Basic. Data were exchanged in real time between the platform and the supervisory system, which was coupled to the bioreactor. It was possible to determine the moment at which maximum enzyme activity was reached in several bioreactor assays. At this point, the end of the process was indicated to the operator. The results illustrate the importance of using reliable computational intelligence-based algorithms in biochemical reactions |
| Disciplinas: | Química |
| Palabras clave: | Química farmacéutica, Biotecnología, Biorreactores, Estimación de estado, Inteligencia artificial, Automatización, Penicilina G acilasa |
| Keyword: | Chemistry, Medicinal chemistry, Biotechnology, Bioreactors, State estimation, Artificial intelligence, Automation, Penicillin G acylase |
| Texto completo: | Texto completo (Ver HTML) |
