| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000391505 |
| ISSN: | 1516-8913 |
| Autores: | Rodríguez, Islay1 Fraga, Jorge1 Noda, Angel Alberto1 Mayet, Milagro2 Duarte, Yanisia3 Echevarria, Eduardo1 Fernández, Carmen1 |
| Instituciones: | 1Instituto de Medicina Tropical "Pedro Kouri", La Habana. Cuba 2Hospital Neumológico Benéfico Jurídico, La Habana. Cuba 3Centro Nacional de Sanidad Agropecuaria, San José de las Lajas, Mayabeque. Cuba |
| Año: | 2014 |
| Periodo: | Jul-Ago |
| Volumen: | 57 |
| Número: | 4 |
| Paginación: | 542-547 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Four variants of the potassium acetate procedure for DNA extraction from ixodid ticks at different stage of their life cycles were evaluated and compared with phenol-chloroform and ammonium hydroxide methods. The most rapid and most efficient variant was validated in the DNA extraction procedure from the engorged ticks collected from bovine, canine as well as from house ticks for the screening of Borrelia burgdorferi sensu lato, Anaplasma spp. and Babesia spp. The ammonium hydroxide procedure was used for non-engorged ticks. All the variants were efficient and allowed obtaining PCR-quality material according to the specific amplification of 16S rRNA gene fragment of the original tick. DNA extracted from the ticks under the study was tested by multiplex PCR for the screening of tick-borne pathogens. Anaplasma spp. and Babesia spp. amplification products were obtained from 29/48 extracts. Ammonium hydroxide protocol was not efficient for two extracts. Detection of amplification products from the PCR indicated that DNA had been successfully extracted. The potassium acetate procedure could be an alternative, rapid, and reliable method for DNA extraction from the ixodid ticks, mainly for poorly-resourced laboratories |
| Disciplinas: | Química, Biología |
| Palabras clave: | Bioquímica, Quelicerados, Acaros, ADN, Extracción química, Reacción en cadena de la polimerasa (PCR) |
| Keyword: | Chemistry, Biology, Biochemistry, Chelicerata, Ticks, DNA, Chemical extraction, Polymerase chain reaction (PCR) |
| Texto completo: | Texto completo (Ver HTML) |
