La inhibición del crecimiento y de la ureasa de Helicobacter pylori por extractos de algas marinas comestibles coreanas
Título del documento: La inhibición del crecimiento y de la ureasa de Helicobacter pylori por extractos de algas marinas comestibles coreanas
Revista: Botanical sciences
Base de datos: PERIÓDICA
Número de sistema: 000374518
ISSN: 2007-4298
Autores: 1
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Instituciones: 1Silla University, Industry-Academic Cooperation Foundation, Busan. Corea del Sur
2Jeju National University, Department of Biology, Jeju. Corea del Sur
3Cyeongnam National University of Science and Technology, Department of Animal Resources Technology, Jinju. Corea del Sur
Año:
Periodo: Dic
Volumen: 91
Número: 4
Paginación: 515-522
País: México
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés Of 27 Korean seaweed species screened for potential anti-H. pylori activity, seven (25.9%) showed strong inhibitory activity based on the agar diffusion method. The strongest activity was observed for ethanol extracts from Ishige okamurae. At 1 mg/disk, the inhibition zone of I. okamurae extract was 9.0 mm, and the minimum inhibitory concentration was 12 µg/ml based on the broth microdilution assay. Based on the free urease assay system, the 80% methanol extracts from I. okamurae had 75.4% inhibition at 0.1 mg/ml. To identify the primary active compounds, I. okamurae powders were successively fractionated according to polarity into five classes of constituents including saccharides, lipids, phenolics, alkaloids, and nitrogen compounds. The I. okamurae phenolic compounds had significant antimicrobial activity (12 µg/ml minimum inhibitory concentration), while the nitrogen compound extract significantly inhibited H. pylori urease activity (80.84% at 1 mg/ml). We evaluated the I. okamurae ethanol and 80% methanol extract for acute toxicity in BALB/c mice. Over the 2-week observation period, no death occurred in any mouse administered a dose of 5 g/kg body weight. These results suggest that I. okamurae extract can be used to develop therapeutic agents for chronic gastritis and peptic ulceration
Disciplinas: Biología,
Medicina
Palabras clave: Algas,
Parasitología,
Medicina experimental,
Helicobacter pylori,
Crecimiento,
Ureasa,
Algas marinas,
Corea,
Alimentos
Keyword: Biology,
Medicine,
Algae,
Parasitology,
Experimental medicine,
Helicobacter pylori,
Growth,
Urease,
Marine algae,
Korea,
Foods
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