High level resistance to phytopathogenic fungi and oomycetes conferred by the use of a novel anti-microbial peptide



Título del documento: High level resistance to phytopathogenic fungi and oomycetes conferred by the use of a novel anti-microbial peptide
Revista: Biotecnología aplicada
Base de datos: PERIÓDICA
Número de sistema: 000348707
ISSN: 0864-4551
Autores: 1
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Instituciones: 1Centro de Ingeniería Genética y Biotecnología, División de Plantas, La Habana. Cuba
2Instituto Nacional de Ciencias Agrícolas, La Habana. Cuba
3Instituto de Investigaciones del Tabaco, San Antonio de los Baños, La Habana. Cuba
Año:
Volumen: 28
Número: 4
Paginación: 262-264
País: Cuba
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Fungi and oomycete-borne plant disease constitute the largest limiting factor for crop productivity and yields both in Cuba and worldwide. Vast amounts of fungicidal chemicals have to be spent every year for their control, despite considerable environmental damage and the risk such chemicals pose for human health. Searching for biotechnological disease control alternatives is, therefore, a priority of current research on this subject. Plant defensins are small cysteine-rich peptides forming part of the defense mechanisms of plants that inhibit the growth of a wide range of phytopathogenic microorganisms. The present work describes a new cDNA coding for a defensin, isolated by looking at genes induced by the inoculation of Nicotiana megalosiphon with Peronospora hyoscyami f. sp. tabacina, the causative pathogen of tobacco blue mould. NmDef02, the isolated gene, was expressed in the yeast Pichia pastoris, and the purified recombinant protein exhibited a strong anti-microbial activity to major fungal and oomycetal plant pathogens. In addition, the constitutive expression of the NmDef02 gene in transgenic tobacco and potato plants increased their resistance to economically important diseases
Disciplinas: Biología,
Agrociencias
Palabras clave: Fitopatología,
Bioquímica,
Hongos fitopatógenos,
Resistencia a plagas,
Plantas transgénicas,
Tabaco,
Papa,
Péptidos antimicrobianos
Keyword: Biology,
Agricultural sciences,
Phytopathology,
Biochemistry,
Phytopathogenic fungi,
Pests resistance,
Transgenic plants,
Tobacco,
Potato,
Antimicrobial peptides
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