Caspase recruitment domains. New potential markers for diagnosis of hepatocellular carcinoma associated with HCV in Egyptian patients
Título del documento: Caspase recruitment domains. New potential markers for diagnosis of hepatocellular carcinoma associated with HCV in Egyptian patients
Revista: Annals of hepatology
Base de datos: PERIÓDICA
Número de sistema: 000415665
ISSN: 1665-2681
Autores: 1
2
3
4
3
3
3
3
Instituciones: 1National Cancer Institute, Virology and Immunology Unit, El Cairo. Egipto
2National Hepatology and Tropical Medicine Research Institute, El Cairo. Egipto
3Cairo University, Kasr El-Aini School of Medicine, El Cairo. Egipto
4National Cancer Institute, Pathology Department, El Cairo. Egipto
Año:
Periodo: Sep-Oct
Volumen: 12
Número: 5
Paginación: 774-781
País: México
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés Chronic HCV is a major cause of HCC development. Caspase Recruitment Domains (CARD) is protein modules that regulate apoptosis and play an important role in various carcinogenesis processes, our aim is to assess the possible role of CARD9, CARD10 and Caspase only protein (COP) in progression of liver fibrosis and pathogenesis of HCC in Egyptian chronic HCV patients. Material and methods. 130 patients were recruited and classified into 4 groups; I: chronic HCV, II: chronic active hepatitis, III: liver cirrhosis, IV: HCV related HCC. Biochemical, virological studies, abdominal ultrasonography and liver biopsy were performed. Quantitative estimation of mRNA of CARD9, CARD10 and COP gene expression was performed by RT- PCR in liver biopsy from all patients. Results. In HCC patients; age, AFP and liver profile were significantly higher, HB and platelets were significantly lower (p value <0.01). The expression levels of mRNA of CARD9, CARD10 and COP in liver biopsies of HCC were significantly higher than other groups with direct correlation with age and no correlation with AFP, viral load, liver fibrosis or necroinflammatory activity. On differentiation between HCC and non HCC patients each CARD was assessed separately and combined, on combing the 3 CARDs, the sensitivity was 100%, specificity was 48%, positive predictive value 47% and negative predictive value 100%. Conclusions. CARD9, CARD10 and COP had no role in liver fibrosis but may be involved in hepatic carcinogenesis and they could be used as markers for HCC diagnosis and candid genes for molecular target therapy
Disciplinas: Medicina
Palabras clave: Diagnóstico,
Gastroenterología,
Oncología,
Virus de la hepatitis C,
Carcinoma hepatocelular,
Marcadores moleculares,
Fibrosis hepática,
Caspasas
Keyword: Medicine,
Diagnosis,
Gastroenterology,
Oncology,
Hepatitis C virus,
Hepatocellular carcinoma,
Molecular markers,
Liver fibrosis,
Caspases
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