Revista: | Annals of hepatology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000416621 |
ISSN: | 1665-2681 |
Autores: | Dolabella, Silvio S1 Serrano Luna, Jesús2 Navarro García, Fernando2 Cerritos, René3 Ximénez, Cecilia3 Galván Moroyoqui, José Manuel2 Silva, Edward F4 Tsutsumi, Víctor2 Shibayama, Mineko2 |
Instituciones: | 1Universidade Federal de Sergipe, Departamento de Morfologia, Aracaju, Sergipe. Brasil 2Instituto Politécnico Nacional, Centro de Investigación y de Estudios Avanzados, México, Distrito Federal. México 3Universidad Nacional Autónoma de México, Facultad de Medicina, México, Distrito Federal. México 4Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas, Belo Horizonte, Minas Gerais. Brasil |
Año: | 2012 |
Periodo: | Ene-Feb |
Volumen: | 11 |
Número: | 1 |
Paginación: | 107-117 |
País: | México |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Analítico, descriptivo |
Resumen en inglés | Although Entamoeba dispar displays a similar morphology to Entamoeba histolytica, cellular and molecular studies have revealed significant differences between these two amoebae, including the former being characterized as non-pathogenic and the later as pathogenic. However, recent in vivo and in vitro experiments have shown that E. dispar strains of different origin are capable of causing liver damage and destroying cell culture lines in the presence of common intestinal bacteria. These results suggested that E. dispar may present pathogenic behavior according to the specific E. dispar strain, culture and environmental conditions. To investigate this possibility, we carried out in vivo and in vitro studies using a xenic strain E. dispar (ICB-ADO) isolated from a symptomatic non-dysenteric Brazilian patient. This strain was able to induce liver necrosis in a hamster model that was more severe than that produced by E. histolytica. The ICB-ADO isolate also caused significantly more destruction of cultured MDCK cells and increased loss of transepithelial resistance than did the E. histolytica. Xenic E. dispar exhibited high proteolytic activity, which was partially inhibited by the addition of cysteine-protease inhibitors. Based on our biochemical and molecular characterization of E. dispar (ICB-ADO) xenic culture and its ability to produce liver abscesses, we conclude that this specific strain can indeed produce tissue damage, distinct from the frequently used non- pathogenic E. dispar SAW 760 strain |
Disciplinas: | Medicina |
Palabras clave: | Gastroenterología, Parasitología, Amibiasis, Absceso hepático, Entamoeba dispar, Entamoeba histolytica, Proteasas |
Keyword: | Gastroenterology, Parasitology, Amebiasis, Liver abscess, Entamoeba dispar, Entamoeba histolytica, Proteases |
Texto completo: | Texto completo (Ver PDF) |