Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments



Título del documento: Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000436159
ISSN: 0001-3765
Autores: 1
1
1
3
2
2
1
2
1
Instituciones: 1Universidade Federal do Parana, Setor de Ciencias Agrarias, Curitiba, Parana. Brasil
2Universidade Federal do Parana, Setor de Ciencias Biologicas, Curitiba, Parana. Brasil
3Fundacao Oswaldo Cruz, Instituto Carlos Chagas, Curitiba, Parana. Brasil
Año:
Volumen: 92
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés In this work, we identified the bacterial microbiota associated with farmed oystersin estuarine regions of four states in the north eastern region of Brazil. During the drought and rainy seasons, for eight months, twenty oysters were sampled seasonally from seven different marine farms. In the laboratory, DNA extraction, amplification, and sequencing of the 16S rRNA gene were performed to establish the taxonomic units. We identified 106 genera of bacteria belonging to 103 families, 70 orders, 39 classes, and 21 phyla. Out of the total, 40 of the genera represented bacteria potentially pathogenic to humans; of these, nine are known to cause foodborne diseases and six are potentially pathogenic to oysters. The most prevalent genera were Mycoplasma, Propionigenium, Psychrilyobacter, and Arcobacter. The results indicate the need for more systematic monitoring of bacteria of the genus Mycoplasma in oyster farming operations in the Brazilian north eastern region. Currently, Mycoplasma is not one of the microorganisms analysed and monitored by order of Brazilian legislation during the oyster production and/or commercialization process, even though this genus was the most prevalent at all sampling points and presents pathogenic potential both for oysters and for consumers
Disciplinas: Medicina veterinaria y zootecnia,
Biología
Palabras clave: Moluscos,
Microbiología,
Granjas ostrícolas,
Estuarios,
Microbiota bacteriana,
Crassostrea
Keyword: Molluscs,
Microbiology,
Oyster farms,
Estuaries,
Bacterial microbiota,
Crassostrea
Texto completo: Texto completo (Ver HTML) Texto completo (Ver PDF)