Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics



Título del documento: Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000420293
ISSN: 0001-3765
Autores: 1
1
1
1
2
1
Instituciones: 1Empresa Brasileira de Pesquisa Agropecuaria, Recursos Geneticos e Biotecnologia, Brasilia, Distrito Federal. Brasil
2Universidade de Sao Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, Sao Paulo. Brasil
Año:
Periodo: Jun
Volumen: 90
Número: 2
Paginación: 1789-1797
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico
Resumen en inglés Brachiaria brizantha is a forage grass well adapted to tropical areas and cultivated in millions of hectares in Brazil. The apomictic mode of reproduction in this species, in addition to differences in ploidy between sexual and apomictic plants, impairs crossbreeding. The development of a methodology to transform apomictic cultivars will provide an option to introduce agronomic important traits to B. brizantha cv. Marandu. In addition, it will open the possibility to study in vivo the function of candidate genes involved in the apomictic reproduction. The objective of this work was to evaluate peeled seeds, isolated embryo from mature seeds, embryogenic calluses and embryogenic cell suspensions, as target explant for genetic transformation via biolistics. Plasmids bearing the marker genes gus and hptII under the control of the rice actin 1 promoter (pAct1-Os) or the maize ubiquitin 1 promoter (pUbi1Zm) were used. All the target- explants used were suitable for transient gene expression after bombardment, showing gus expression and resistance to hygromycin. Using embryogenic calluses and cell suspensions as target tissues, transgenic plants were regenerated and transgenes detected
Disciplinas: Agrociencias,
Medicina veterinaria y zootecnia
Palabras clave: Fitotecnia,
Genética,
Nutrición animal,
Forraje,
Gen gus,
Gen HptII,
Higromicina,
Brachiaria brizantha
Keyword: Crop husbandry,
Genetics,
Animal nutrition,
Forage,
Gus gene,
HptII gene,
Hygromycin,
Brachiaria brizantha
Texto completo: Texto completo (Ver PDF)