| Revista: | Anais da Academia Brasileira de Ciencias |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000417626 |
| ISSN: | 0001-3765 |
| Autores: | Silva, Monique O.M1 Armada, Jorge Luis A. de1 Verona, Carlos Eduardo S2 Heliodoro, Gabriela3 Nogueira, Denise M1 |
| Instituciones: | 1Universidade Federal Rural do Rio de Janeiro, Instituto de Ciencias Biologicas e da Saude, Seropedica, Rio de Janeiro. Brasil 2Instituto Triade, Instituto Brasileiro para Medicina da Conservacao, Recife, Pernambuco. Brasil 3Jardim Botanico do Rio de Janeiro, Instituto de Pesquisas, Rio de Janeiro. Brasil |
| Año: | 2017 |
| Periodo: | Dic |
| Volumen: | 89 |
| Número: | 4 |
| Paginación: | 2793-2804 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A fragment of approximately 200bp of the SRY gene was amplified in seven females with normal external genitalia. The percentage of 32% of chimeric individuals detected in the present study is similar to that observed for pure specimens of Callithrix. These data suggests that hybridization probably does not interfere with the occurrence of twin gestation, nor of chimerism. Although cytogenetics is the main tool to identify the two cell lineages present in cases of chimerism, the amplification of the SRY gene by PCR has proved to be more efficient to identify the Y chromosome in cases of chimeric female marmoset |
| Disciplinas: | Biología |
| Palabras clave: | Mamíferos, Genética, Análisis citogenético, Genética molecular, Cariotipos, Quimerismo, Hibridación genética, Callithrix |
| Keyword: | Mammals, Genetics, Cytogenetic analysis, Molecular genetics, Karyotypes, Chimerism, Genetic hybridation, Callithrix |
| Texto completo: | Texto completo (Ver HTML) |
