Revista: | Anais da Academia Brasileira de Ciencias |
Base de datos: | PERIÓDICA |
Número de sistema: | 000424364 |
ISSN: | 0001-3765 |
Autores: | Wei, Tao1 Yang, Kunpeng1 Zang, Jie1 Mao, Duobin1 |
Instituciones: | 1Zhengzhou University of Light Industry, School of Food and Biological Engineering, Zhengzhou, Henan. China |
Año: | 2018 |
Periodo: | Sep |
Volumen: | 90 |
Número: | 3 |
Paginación: | 2731-2740 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | The DNA nuclease gene ST2109 has been cloned from the hyperthermophilic archaeon Sulfolobus tokodaii and expressed in Escherichia coli. The recombinant protein StoNurA has been purified to homogeneity by affinity chromatography and gel filtration chromatography. Biochemical analyses demonstrated that StoNurA exhibited DNA binding and 5’–3’ exonuclease activities towards ssDNA and dsDNA. The temperature and pH optima of StoNurA were determined to be 65 °C and 8.0, respectively. The activity of StoNurA was found to be dependent of Mn2+, and its half-life of heat inactivation at 100 °C was 5 min. Gel filtration chromatography revealed that StoNurA could form dimers in solution. Pull-down assays also showed that StoNurA physically interacted with a DNA helicase (StoHerA). Our data suggest that NurA may play a key functional role in the processing of DNA recombinational repair |
Disciplinas: | Biología |
Palabras clave: | Genética, Bioquímica, ADN nucleasa, Genes, Reparación genética, Recombinación genética, Dímeros, Sulfolobus tokodaii |
Keyword: | Genetics, Biochemistry, DNA nuclease, Genes, Genetic repairment, Genetic recombination, Dimers, Sulfolobus tokodaii |
Texto completo: | Texto completo (Ver HTML) |