Dynamics of proteins, carbohydrates and global DNA methylation patterns during induction of nodular cluster cultures from seeds of Vriesea reitzii
Título del documento: Dynamics of proteins, carbohydrates and global DNA methylation patterns during induction of nodular cluster cultures from seeds of Vriesea reitzii
Revista: Acta scientiarum. Agronomy
Base de datos: PERIÓDICA
Número de sistema: 000460006
ISSN: 1679-9275
Autores: 1
2
2
2
2
Instituciones: 1Universidad de Sucre, Departamento de Biología y Química, Sincelejo, Sucre. Colombia
2Universidade Federal de Santa Catarina, Florianopolis, Santa Catarina. Brasil
3Universidade Federal de Santa Catarina, Centro de Ciencias Agrarias, Curitibanos, Santa Catarina. Brasil
Año:
Volumen: 42
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés Tissue culture techniques have been employed for bromeliad mass propagation by means of the morphogenetic route of nodular cluster cultures (NCs). This study aimed to assess protein, carbohydrate and global DNA methylation (GDM) level dynamics during NCs induction from Vriesea reitzii seeds. Seeds were inoculated into Murashige and Skoog (MS) liquid medium supplemented with 4 µM α-naphthaleneacetic acid (NAA) to induce NCs and in culture medium without plant growth regulators to form normal seedlings. Samples collected at 0, 3, 7, 10, 14, and 21 days of culture were analyzed. All parameters assessed showed the same variation pattern. However, seeds inducing NCs showed significantly lower starch (6.0 mg g-1 FM), carbohydrate (10.7 mg g-1 FM) and GDM (11.0%) levels than seeds forming normal seedlings after 21 days in culture. On the other hand, the protein content (9.1 mg g-1 FM) was significantly higher during induction. NCs induction process through seeds is the result of gene reprogramming in the explant, which leads to morphological, biochemical and metabolic alterations. This involves dedifferentiation, high cell proliferation, high energy demand and protein synthesis, which is related to elevated metabolic activity
Disciplinas: Química,
Biología
Palabras clave: Fitoquímica,
Angiospermas,
Genética,
Vriesea reitzii,
Bromeliaceae,
ADN,
Germinación,
Micropropagación vegetal
Keyword: Phytochemistry,
Angiosperms,
Genetics,
Vriesea reitzii,
Bromeliaceae,
Germination,
Micropropagation
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