| Revista: | Acta bioquímica clínica latinoamericana |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000365275 |
| ISSN: | 0325-2957 |
| Autores: | Madalena, Leticia1 Pandolfo, Marcela1 Facio, María Laura1 Garlatti, Claudia1 Alejandre, Mariel1 Bresciani, Pablo Diego1 Angerosa, Margarita1 Pizzolato, Marco1 |
| Instituciones: | 1Universidad de Buenos Aires, Hospital de Clínicas "José de San Martín", Buenos Aires. Argentina |
| Año: | 2013 |
| Periodo: | Mar |
| Volumen: | 47 |
| Número: | 1 |
| Paginación: | 85-93 |
| País: | Argentina |
| Idioma: | Español |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en español | La proteinuria (PT-ur) es un marcador de enfermedad renal, sin un método de referencia establecido. Mediante electroforesis en gel de poliacrilamida con dodecilsulfato de sodio (SDS-PAGE) con tinción argéntica pueden identificarse de manera no-invasiva perfiles proteicos urinarios (PPU) de tipo glomerular (G), tubular (Tc: completos, Ti: incompletos) y mixtos (GTc, GTi) con fines diagnósticos/pronósticos y de evolución. El objetivo del estudio fue determinar la prevalencia (P) de los PPU para cada nivel de PT-ur obtenido con cuatro métodos diferentes, en pacientes con sospecha de lesión renal. Se procesaron 105 orinas de 24 h de recolección clasificadas mediante tira reactiva (TR) y 2 pacientes con proteinuria de Bence Jones (PBJ)>2 g/L. La cuantificación de PT-ur se realizó con ácido sulfosalicílico (SSA) y Bradford (CBB) “in-house”; cloruro de benzetonio (BZT-Roche) y rojo de pirogallol-molibdato (PRM-Wiener)-Hitachi 917. Se realizó uroproteinograma y SDS-PAGE. Los cuatro métodos presentaron correlación significativa (p<0,05) para PT-ur y una comparación de medias estadísticamente no-significativa en todos los niveles de TR. Para TR negativa (PT-ur en rango normal), la P del Ti fue del 33% y del 16% para GTi. En TR 1+/2+, el GTi de mediana selectividad presentó una P del 33%/30% y el GTc de 26%/57%, respectivamente, que se incrementó al 66% para TR 3+, con escasa-selectividad. Las PBJ presentaron valores estables mediante BZT, con diluciones manuales o realizadas por el equipo. La PT-ur mediante los métodos de cuantificación ensayados resultaron comparables y el BZT mostró PT-ur variables en presencia de PBJ dependiendo de la dilución empleada. En todos los niveles de PT-ur fue posible identificar la P de todas las lesiones estructurales con selectividades progresivamente comprometidas |
| Resumen en inglés | Proteinuria (TP-ur) is associated with adverse outcomes. Analytical specificities as well as the calibration of the methods cause considerable variation of TP-ur. SDS-PAGE with silver staining is an excellent single-test for non-invasively diagnosing a complete (cT) or incompleted tubular (iT) and/or glomerular (G) protein profile (PP). In order to establish the prevalence (P) of structural lesions and to compare four TP-ur methods, one hundred and five 24 h urine collection, selected by dipstick urinalysis values (DUV), were analyzed. Coomassie Brilliant Blue G-250(CBB)/Sulphosalicylic Acid (SSA) (in-house manually developed methods) and benzethonium-chloride(BZT)/ pyrogallol red-molybdate(PRM) (Hitachi-917 analyzer) were employed. Two samples containing >2 g/L of Bence Jones protein (BJP) were assayed. SDS-PAGE and conventional zone electrophoresis (ZE), both stained with the silver stain procedure were developed. The data showed consistency in detecting a P of GiT (16%) and iT (33%) for negative DUV. 1+/2+ DUV presented a P of 33%, 30% for GiT with a partially conserved selectivity, and 26%, 57% of a GcT PP, respectively. In 3+ DUV a GcT PP was 66%, with non-selective profile. Statistically significant correlation coefficients (p<0.05) were observed for all methods in a range of 0-10.0 g/L of TP-ur, with no significant differences between TP-ur means. BJP reached a stable value with BZT, manually or automatized diluted. In conclusion, investigating the proteinuria P by means of SDS-PAGE provided useful information on the underlying structural renal problem in the samples analyzed for all the methods employed in different TP-ur levels. When BJP >2 g/L TP-ur must be performed by diluting the sample to get useful clinical information |
| Resumen en portugués | Proteinuria (TP-ur) is associated with adverse outcomes. Analytical specificities as well as the calibration of the methods cause considerable variation of TP-ur. SDS-PAGE with silver staining is an excellent single-test for non-invasively diagnosing a complete (cT) or incompleted tubular (iT) and/or glomerular (G) protein profile (PP). In order to establish the prevalence (P) of structural lesions and to compare four TP-ur methods, one hundred and five 24 h urine collection, selected by dipstick urinalysis values (DUV), were analyzed. Coomassie Brilliant Blue G-250(CBB)/Sulphosalicylic Acid (SSA) (in-house manually developed methods) and benzethonium-chloride(BZT)/ pyrogallol red-molybdate(PRM) (Hitachi-917 analyzer) were employed. Two samples containing >2 g/L of Bence Jones protein (BJP) were assayed. SDS-PAGE and conventional zone electrophoresis (ZE), both stained with the silver stain procedure were developed. The data showed consistency in detecting a P of GiT (16%) and iT (33%) for negative DUV. 1+/2+ DUV presented a P of 33%, 30% for GiT with a partially conserved selectivity, and 26%, 57% of a GcT PP, respectively. In 3+ DUV a GcT PP was 66%, with non-selective profile. Statistically significant correlation coefficients (p<0.05) were observed for all methods in a range of 0-10.0 g/L of TP-ur, with no significant differences between TP-ur means. BJP reached a stable value with BZT, manually or automatized diluted. In conclusion, investigating the proteinuria P by means of SDS-PAGE provided useful information on the underlying structural renal problem in the samples analyzed for all the methods employed in different TP-ur levels. When BJP >2 g/L TP-ur must be performed by diluting the sample to get useful clinical information |
| Disciplinas: | Química, Medicina |
| Palabras clave: | Bioquímica, Nefrología, Proteinuria |
| Keyword: | Chemistry, Medicine, Biochemistry, Nephrology, Proteinuria |
| Texto completo: | Texto completo (Ver PDF) |
