| Revista: | Universitas scientiarum |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000360982 |
| ISSN: | 0122-7483 |
| Autors: | Tovar Franco, Jairo Alfonso1 Aparicio Castellanos, Diana Constanza2 Henríquez Higuera, Luisa Fernanda2 |
| Institucions: | 1Pontificia Universidad Javeriana, Departamento de Nutrición y Bioquímica, Bogotá. Colombia 2Pontificia Universidad Javeriana, Departamento de Odontología, Bogotá. Colombia |
| Any: | 2009 |
| Període: | Ene-Abr |
| Volum: | 14 |
| Número: | 1 |
| Paginació: | 58-70 |
| País: | Colombia |
| Idioma: | Español |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en español | El principal problema que recae sobre el odontólogo en la toma de evidencias post mortem es la apertura bucal, limitada por el fenómeno de rigidez cadavérica el cual inicia a las 3 horas después de la muerte, siendo éste un fenómeno que persiste hasta ser destruido por los procesos autolíticos tardíos de descomposición, a partir de las 36 horas post mortem. Objetivo. Se estudió la posibilidad de acelerar la reversión de la rigidez cadavérica mandibular por medio de sustancias químicas para facilitar la apertura bucal. Materiales y métodos. Se encaminó al uso de sustancias que produzcan la alteración del pH, quelación del calcio intramuscular o proteólisis del complejo actina-miosina de los músculos masticatorios que presenten rigidez cadavérica en ratas Wistar. Resultados. Se determinó que el tiempo de establecimiento de la rigidez cadavérica mandibular en el modelo de rata, fue de 2,5 horas. A las 3,5 horas, una vez establecida la rigidez, se realizaron infiltraciones con EDTA (20 mM), NaHCO3 (50 µM), Na2CO3 (50 µM) y papaína (10 µM) encontrándose que las soluciones de NaHCO3 y Na2CO3 incrementaron significativamente (p<0,05) la velocidad de reversión (mm/h) desde las 5 h, en un 108% y un 100%, respectivamente. A partir de este ensayo se duplicó la concentración de NaHCO3 y se preparó una mezcla de NaHCO3 y Na2CO3 (1:1) sin encontrar diferencias significativas con los ensayos con NaHCO3 y Na2CO3. Conclusión. La solución de NaHCO3 (50 µM) logró revertir la apertura bucal suficiente para la toma de evidencia entre las 5- 5,5 horas |
| Resumen en inglés | The main problem that a dentist faces when collecting postmortem evidence is the buccal opening, which is limited by the cadaverous stiffness phenomenon that begins 3 hours after death. This phenomenon persists until it is destroyed by the late autolytic processes of decomposition, after 36 hours postmortem. Objective. To analyze the feasibility of accelerating the reversion of the mandibular cadaverous stiffness by means of chemical substances to facilitate the buccal opening. Materials and methods. We assessed substances capable of altering the pH, chelating intramuscular calcium or inducing proteolysis of the actin-myosin complex of the masticatory muscles with cadaverous stiffness in Wistar rats. Results. We found that mandibular cadaverous stiffness in Wistar rats appears after 2.5 hours of death. After 3.5 hours, once the rigidity was established, we carried out infiltrations with EDTA (20 mM), NaHCO3 (50 µM), Na2CO3 (50 µM) and papain (10 µM). NaHCO3 and Na2CO3 solutions significantly increased (p<0.05) the reversion speed (mm/h) from hour 5, in 108% and 100%, respectively. Based on the results of this assay, we doubled the concentration of NaHCO3 and assessed a 1:1 mixture of NaHCO3 and Na2CO3 without finding significant differences with the NaHCO3 and Na2CO3 prior assays. Conclusion. NaHCO3 solution (50 µM) allows a reversal of buccal opening enough to collect evidence between 5 and 5.5 hours |
| Resumen en portugués | The main problem that a dentist faces when collecting postmortem evidence is the buccal opening, which is limited by the cadaverous stiffness phenomenon that begins 3 hours after death. This phenomenon persists until it is destroyed by the late autolytic processes of decomposition, after 36 hours postmortem. Objective. To analyze the feasibility of accelerating the reversion of the mandibular cadaverous stiffness by means of chemical substances to facilitate the buccal opening. Materials and methods. We assessed substances capable of altering the pH, chelating intramuscular calcium or inducing proteolysis of the actin-myosin complex of the masticatory muscles with cadaverous stiffness in Wistar rats. Results. We found that mandibular cadaverous stiffness in Wistar rats appears after 2.5 hours of death. After 3.5 hours, once the rigidity was established, we carried out infiltrations with EDTA (20 mM), NaHCO3 (50 µM), Na2CO3 (50 µM) and papain (10 µM). NaHCO3 and Na2CO3 solutions significantly increased (p<0.05) the reversion speed (mm/h) from hour 5, in 108% and 100%, respectively. Based on the results of this assay, we doubled the concentration of NaHCO3 and assessed a 1:1 mixture of NaHCO3 and Na2CO3 without finding significant differences with the NaHCO3 and Na2CO3 prior assays. Conclusion. NaHCO3 solution (50 µM) allows a reversal of buccal opening enough to collect evidence between 5 and 5.5 hours |
| Disciplines | Medicina |
| Paraules clau: | Medicina experimental, Odontología, Apertura bucal, Rigor mortis, Músculos masticadores, Medicina forense |
| Keyword: | Medicine, Dentistry, Experimental medicine, Buccal opening, Rigor mortis, Masticatory muscles, Forensic medicine |
| Text complet: | Texto completo (Ver HTML) |
