The action of red scorpion (Mesobuthus tamulus coconsis, pocock) venom and its isolated protein fractions on blood sodium levels
Título del documento: The action of red scorpion (Mesobuthus tamulus coconsis, pocock) venom and its isolated protein fractions on blood sodium levels
Revista: The journal of venomous animals and toxins including tropical diseases
Base de datos: PERIÓDICA
Número de sistema: 000299002
ISSN: 1678-9199
Autores: 1


2
Instituciones: 1Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Department of Pharmaceutical Chemistry, Pune, Maharashtra. India
2Haffkine Biopharmaceutical Corporation Ltd., Pune, Maharashtra. India
Año:
Volumen: 13
Número: 1
Paginación: 82-93
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés Red scorpion (Mesobuthus tamulus or Buthus tamulus) venom samples were collected at different regions of India: western (Chiplun and Ahmednagar from Maharashtra State) and southern (Ratnagiri and Chennai from Tamil Nadu State). The action of whole venoms on the blood sodium levels of mice was assessed using flame photometry. Seven peptides were common to all venom samples. They were separated using the native polyacrylamide gel electrophoresis (PAGE) technique and their activities were also studied using flame photometry. There was a decrease in the concentration of sodium ions in the serum, which suggested the blockage of such ions by scorpion venom toxins. Among the 10 protein bands isolated, the band at 79.6 kDa presented maximum activity in decreasing serum sodium ions concentration. Whole venom from Chiplun region also showed maximum activity. The western blotting technique demonstrated that the anti-scorpion venom sera produced by Haffkine Biopharmaceuticals Corporation Ltd., India, neutralized all four venom samples
Disciplinas: Medicina
Palabras clave: Farmacología,
Toxicología,
Veneno de escorpión,
Fracción protéica,
Sangre,
Iones de sodio,
Buthus tamulus,
Buthidae
Keyword: Medicine,
Pharmacology,
Toxicology,
Scorpion venom,
Protein fractions,
Blood,
Sodium ions,
Buthus tamulus,
Buthidae
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