| Revista: | Scientia agricola |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000283996 |
| ISSN: | 0103-9016 |
| Autores: | Broetto, F1 Casa, A.M Malavolta, E2 Lopes, C.R |
| Instituciones: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Instituto de Biociencias, Botucatu, Sao Paulo. Brasil 2Universidade de Sao Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, Sao Paulo. Brasil |
| Año: | 1997 |
| Volumen: | 54 |
| Número: | 3 |
| Paginación: | 128-132 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | One of the uses of the technique of tissue culture for plant breeding is the identification of cell lines tolerant to salt stress.In order to study the biochemical mechanisms involved in the genetic expression to salt tolerance, callus from embryo axis of four bean cultivars (cv. IAC-carioca; cv. IAPAR-14; cv. JALO-EEP558; CV. BAT-93) were grown in Murashige & Skoog (1962) medium, supplemented with NaCl in the concentrations of 0, 20, 40, 60 and 80 mM. After 14 days callus were harvested and analyzed according to their isoenzymatic patterns and peroxidase activities. BAT and IAPAR cultivars showed two common activity zones in the anodic region, with only one specific enzymatic band to each one (the two fastest migration band); it is possible that the two middle anodic zones detected are products of the same enzymatic locus but from different alleles with different eletrophoretic mobilities. Cv. JALO showed two anodic activities in common with cvs IAC and IAPAR with an exclusive anodic zone of slower migration which showed the most intense activity of all cultivars analyzed. This cv. still showed a dimeric heterozygotic catodic zone in all treated samples. Probably this is the same zone which occurs in homozygosis with fixation of the slower allele for all cvs BAT and IAPAR submitted to all treatments. Cv. IAC showed two anodic bands in common with Cv. IAPAR and cv. JALO. It still showed a faster anodic band in common with cv. IAPAR and an exclusive anodic band of slower migration. It is interesting to say that for this cv |
| Disciplinas: | Agrociencias |
| Palabras clave: | Leguminosas, Biotecnología, Frijol, Phaseolus vulgaris, Peroxidasa, Polimorfismo, Estrés salino, Salt stress |
| Keyword: | Agricultural sciences, Legumes, Phaseolus vulgaris, Peroxidase, Bean, Polymorphism |
| Texto completo: | Texto completo (Ver HTML) |
