| Revista: | Scientia agricola |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000359323 |
| ISSN: | 0103-9016 |
| Autors: | Sousa, Adna Cristina Barbosa de1 Godoy, Rodolfo2 Sforca, Danilo Augusto1 Campos, Tatiana de1 Zucchi, Maria Imaculada3 Jank, Liana4 Souza, Anete Pereira de1 |
| Institucions: | 1Universidade Estadual de Campinas, Centro de Biologia Molecular e Engenharia Genetica, Campinas, Sao Paulo. Brasil 2Empresa Brasileira de Pesquisa Agropecuaria, Pecuaria Sudeste, Sao Carlos, Sao Paulo. Brasil 3Instituto Agronomico, Polo Apta Sul, Piracicaba, Sao Paulo. Brasil 4Empresa Brasileira de Pesquisa Agropecuaria, Gado de Corte, Campo Grande, Mato Grosso do Sul. Brasil |
| Any: | 2011 |
| Període: | Jul-Ago |
| Volum: | 68 |
| Número: | 4 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico |
| Resumen en inglés | The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47, respectively, showing a deficit in heterozygosity. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign genotypes into clusters. A dendrogram was constructed based on the modified Roger's genetic distances using a neighbor-joining method (NJ). A total of four clusters were assembled by STRUCTURE and a strong tendency of correspondence between the Bayesian clusters in the NJ tree was observed. The genetic distance ranged from 0.09 to 0.62 (average 0.37), showing a low genetic diversity in the pigeonpea genotypes. Transferability of pigeonpea-specific microsatellites revealed a cross-amplification and the presence of polymorphic alleles in P. vulgaris and V. unguiculata |
| Disciplines | Agrociencias |
| Paraules clau: | Leguminosas, Genética, Gandul, Cajanus cajan, Germoplasma, Diversidad genética, Microsatélites, Marcadores genéticos, Genotipos, Mejoramiento genético, América del Sur |
| Keyword: | Agricultural sciences, Legumes, Genetics, Pigeon pea, Cajanus cajan, Germplasm, Genetic diversity, Microsatellites, Genetic markers, Genotypes, Plant breeding, South America |
| Text complet: | Texto completo (Ver HTML) |
