| Revista: | Revista médica del Hospital General de México |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000439515 |
| ISSN: | 0185-1063 |
| Autors: | Alemán García, Nathalie1 Sánchez Pérez, Celia1 Pérez García, Adolfo2 Durán Padilla, Marco A3 Kershenobich, David4 Hernández Ruiz, Joselín5 |
| Institucions: | 1Universidad Nacional Autónoma de México, Instituto de Ciencias Aplicadas y Tecnología, Ciudad de México. México 2Hospital General de México, Servicio de Cirugía Experimental, Ciudad de México. México 3Hospital General de México, Servicio de Patología, Ciudad de México. México 4Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México. México 5Universidad Nacional Autónoma de México, Facultad de Medicina, Ciudad de México. México |
| Any: | 2020 |
| Període: | Ene-Mar |
| Volum: | 83 |
| Número: | 1 |
| Paginació: | 13-19 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Animal models for hepatic fibrosis are currently used for clinical studies, and research works aimed to the progression and regression of the disease. In these, fibrosis is evaluated using histopathological assessment with high variability inter- and intra-observers. Digital morphometry in the space color LAB could reduce this variability; however, its correlation degree with the histopathological assessment is unknown. Objective: The objective of the study was to analyze the correlation between digital morphometry and the histopathological assessment of hepatic fibrosis in images of biopsies of a murine model. Materials and methods: We performed a descriptive, experimental, longitudinal, prospective, analytical, and correlational study. Fibrosis was induced by intraperitoneal injection of carbon tetrachloride in 16 Wistar male rats. We did a postmortem hepatectomy and took histological specimens stained by Masson trichrome, obtaining ten randomly selected images at ×10 in each liver. We quantified the fibrosis percentage (FP) by digital morphometry and compared it to the Metavir score given by three independent evaluators. FP was analyzed by a ANOVA test and Spearman Rho test. Results: The evaluated images had 70% concordance of the three evaluators. The analysis of the FP revealed fibrosis evaluation groups with statistical differences. The FP had a very high correlation with the histopathological diagnosis (p = 0.0001, r = 0-9). The ranges obtained from FP for each phase of fibrosis were: 0.4% for F0, 0.44-1.14% for F1, 1.52-2.74% for F2, 3.1-3.8% for F3y, and 5.12-6.12% for F4. Conclusions: The morphometric quantification had a high correlation with the histopathological evaluation of images of histological sections of liver biopsies of the murine model of fibrosis. The quantification of the FP by digital morphometry will be useful to increase the accuracy in the evaluation of liver fibrosis |
| Disciplines | Medicina |
| Paraules clau: | Gastroenterología, Medicina experimental, Fibrosis hepática, Modelos animales, Modelo murino, Morfometría digital |
| Keyword: | Gastroenterology, Experimental medicine, Liver fibrosis, Animal models, Murine model, Digital morphometry |
| Text complet: | https://www.hospitalgeneral.mx/frame_esp.php?id=48 |
