| Revista: | Revista do Instituto de Medicina Tropical de Sao Paulo |
| Base de datos: | |
| Número de sistema: | 000547851 |
| ISSN: | 0036-4665 |
| Autors: | Canché Pool, Elsy Beatriz1 Canto Hau, Dulce María1 Vargas Meléndez, Mirna Alexandra1 Tello Martín, Raúl1 Reyes Novelo, Enrique1 Escobedo Ortegón, Francisco Javier1 Ruiz Piña, Hugo Antonio1 Cambranes Puc, Luis Humberto1 Torres Castro, Jimmy Raymundo2 Palacio Vargas, Jorge Alfredo2 Durán Caamal, Celmy2 Cerón Espinosa, José3 Carpio Pedroza, Juan Carlos4 Rivera Hernández, Octavio César4 |
| Institucions: | 1Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Mérida, Yucatán. México 2Servicios de Salud de Yucatán, Dirección de Prevención y Protección de la Salud, Mérida, Yucatán. México 3Centro Dermatológico de Yucatán, Servicios de Salud de Yucatán, Mérida, Yucatán. México 4Instituto de Diagnóstico y Referencia Epidemiológicos, Departamento de Parasitología, Ciudad de México. México |
| Any: | 2022 |
| Volum: | 64 |
| País: | Brasil |
| Idioma: | Inglés |
| Resumen en inglés | Localized cutaneous leishmaniasis (LCL) is an endemic disease in several Mexican States with the main endemic areas located in the South-Southeast region of the country, where 90% of Leishmania (Leishmania) mexicana cases are registered. The Southeast region is located in the Yucatan Peninsula, including Campeche, Quintana Roo and Yucatan States. Campeche and Quintana Roo register more than 60% of the cases in the country each year, while in Yucatan the reports are of imported cases due to residents traveling to endemic areas. However, since 2015, autochthonous cases have been diagnosed by health authorities in municipalities with no previous transmission records. We aimed to identify Leishmania parasite species involved in autochthonous cases by means of the PCR technique. The present study included 13 autochthonous cases of LCL with clinical and parasitological diagnoses during 2018 and 2019 by health authorities, without specific identification of the causal agent. Tissue samples were taken by scraping the margins of active lesions and then they were spotted onto an FTATM Elute Microcard. Next, DNA was eluted and used for PCR amplification of specific Leishmania genus and L. (L.) mexicana species-specific fragments. Molecular analysis showed evidence that L. (L.) mexicana was the causal agent of LCL in 12 of the 13 patients; in one patient, PCR was not performed due to the patient’s refusal to participate in the study. Identifying Leishmania species that cause LCL is necessary to define efficient treatment schemes and control strategies for the disease in vulnerable and susceptible areas of the Yucatan State’s municipalities. |
| Keyword: | Leishmaniasis, Leishmania (Leishmania) mexicana, Autochthonous cases, Emergence, Neglected tropical diseases, Species-specific PCR |
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