Characterization of selection effects on broiler lines using DNA fingerprinting



Título del documento: Characterization of selection effects on broiler lines using DNA fingerprinting
Revista: Revista brasileira de ciencia avicola
Base de datos: PERIÓDICA
Número de sistema: 000279211
ISSN: 1516-635X
Autors: 1
1
Institucions: 1Empresa Brasileira de Pesquisa Agropecuaria, Centro Nacional de Pesquisa de Suinos e Aves, Concordia, Santa Catarina. Brasil
Any:
Període: May-Ago
Volum: 5
Número: 2
Paginació: 119-123
País: Brasil
Idioma: Inglés
Tipo de documento: Nota breve o noticia
Enfoque: Experimental
Resumen en inglés The objective of this study was to evaluate the effect of selection for body weight on the genetic variability and diversity in broiler lines. Two paternal broiler lines (LL and LLc) were used. LL line was selected for 12 generations for growth and carcass and reproduction characteristics. The LLc line was established from LL line in 1985 and mated at random. Blood samples from six chickens per line were collected and used for molecular analysis. Also, a DNA pool was made for each line to compare effects between lines. Data were analyzed considering the collected information on the presence or absence of DNA bands. Band sharing scores were calculated using the DICE coefficient. The pattern of the 21 most representative bands was used. DNA fingerprinting (DFP) showed 90.48 % of polymorphism bands for both lines. Difference between lines was not due to the presence or absence of bands, but to the frequency of such bands in each genotype. Considering that both lines had the same genetic background, changes on band frequency were probably due to selection. Selection for body weight had an effect on the band frequency as evaluated by DFP, and for this reason this technique could be used as a tool in the selection process. Results also suggest that bands 4, 5 and 19 were linked to body weight traits, and bands 9, 10, 12, 13 and 21 were linked to reproductive traits such as egg production
Disciplines Medicina veterinaria y zootecnia,
Biología
Paraules clau: Aves de corral,
Biología molecular,
Pollos de engorda,
Variabilidad genética,
Selección,
Marcadores moleculares,
ADN,
Peso corporal
Keyword: Veterinary medicine and animal husbandry,
Biology,
Poultry,
Molecular biology,
Broilers,
Genetic variability,
Selection,
Molecular markers,
DNA,
Body weight
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