| Revista: | Quimica nova |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000313153 |
| ISSN: | 0100-4042 |
| Autors: | Catharino, Rodrigo Ramos1 Godoy, Helena Teixeira Lima-Pallone, Juliana Azevedo2 |
| Institucions: | 1Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos, Campinas, Sao Paulo. Brasil 2Pontificia Universidade Catolica de Campinas, Faculdade de Quimica, Campinas, Sao Paulo. Brasil |
| Any: | 2006 |
| Període: | Sep |
| Volum: | 29 |
| Número: | 5 |
| Paginació: | 972-976 |
| País: | Brasil |
| Idioma: | Portugués |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The aim of this work was to develop and to validate a methodology using HPLC for the simultaneous determination of folates and folic acid in foods. The limits of detection and the recovery rates for the vitamins in the certified reference materials were respectively 5 pg/mL and 94-108% for 5-MTHF, 7 pg/mL and 97-102% for THF, 30 pg/mL and 97.9-104% for 5-FTHF, 30 pg/mL and 95-107 for 10-FFA, 5 ng/mL and 97-102% for FA and 5 ng/mL and 98-103% for 10-MFA. Repeatability showed a coefficient of variation below 3.9% for all the vitamins. The proposed methodology was shown to be efficient when applied to different certified reference materials, namely pig's liver (BCR487), powdered milk (BCR421) and a vegetable mixture (BCR485) |
| Disciplines | Química |
| Paraules clau: | Química analítica, Química de alimentos, Cromatografía líquida de alta resolución (HPLC), Folatos, Acido fólico |
| Keyword: | Chemistry, Analytical chemistry, Food chemistry, High performance liquid chromatography (HPLC), Folates, Folic acid |
| Text complet: | Texto completo (Ver HTML) |
