| Revista: | Pesquisa veterinaria brasileira |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000301585 |
| ISSN: | 0100-736X |
| Autores: | Diel, Diego Gustavo1 Fonseca, Erika Toledo da Souza, Soraia Figueiredo de Mazzanti, Alexandre1 Bauermann, Fernando Weiblen, Rudi Flores, Eduardo Furtado |
| Instituciones: | 1Universidade Federal de Santa Maria, Departamento de Clinica de Pequenos Animais, Santa Maria, Rio Grande do Sul. Brasil |
| Año: | 2005 |
| Periodo: | Jul-Sep |
| Volumen: | 25 |
| Número: | 3 |
| Paginación: | 164-170 |
| País: | Brasil |
| Idioma: | Portugués |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Bovine herpesvirus type 5 (BoHV-5) is a major etiological agent of meningoencephalitis in cattle. Following replication in the nasal mucosa, viral invasion of the brain is thought to occur mainly by the olfactory pathway. To address the role of this pathway in the pathogenesis of neurological infection in a laboratory model, 30 days old rabbits had the main olfactory bulbs (MOBs) surgically removed and were subsequently inoculated intranasally (IN) or conjunctivally (IC) with a highly neurovirulent BoHV-5 strain (SV-507). Following IN inoculation, 10 out of 10 (100 %) control rabbits developed neurological disease. The clinical onset ranged from day 5 to 10 post-inoculation (pi, average 7.5 days); nine being euthanized in extremis and one recovering after a mild clinical course. In contrast, only one rabbit (9.1 %) of the group lacking the MOBs (n=11) developed neurological disease (onset at day 17 pi). Dexamethasone administration to the survivors (n=10) at day 50pi was followed by virus shedding in nasal and/or ocular secretions by 8 animals, demonstrating that the virus was able to reach the trigeminal ganglia (TG) during acute infection. These results demonstrate that the olfactory route provides the main, yet not the sole access to the brain of rabbits following IN inoculation. To address the role of a second pathway, groups of control (n=12) or MOB-lacking rabbits (n=12) were inoculated into the conjunctival sac (IC), following which the virus would be expected to use the ophtalmic branch of the trigeminal nerve to reach the brain. Ten control rabbits (83.3 %) developed neurological disease upon IC inoculation (onset 15.3 days 11 to 20). Previous ablation of the MOBs did not affect the frequency and course of neurological disease: ten out of 12 rabbits (83.3 %) lacking the MOBs developed neurological disease (onset 9 to 15 dpi, average: 12.7 days) upon IC inoculation. These results demonstrate that both IN and IC routes may operate in the transport of B |
| Disciplinas: | Medicina veterinaria y zootecnia, Biología |
| Palabras clave: | Medicina veterinaria, Otras especies de interés zootécnico, Virus, Herpesvirus bovino tipo 5 (BHV-5), Conejos, Infecciones neurológicas |
| Keyword: | Veterinary medicine and animal husbandry, Biology, Species of commercial interest, Veterinary medicine, Virus, Rabbits, Bovine herpesvirus type 5 (BHV-5), Neurological infections |
| Texto completo: | Texto completo (Ver HTML) |
