| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000405458 |
| ISSN: | 0074-0276 |
| Autors: | Santana, Nadja de Lima2 Rego, Jamile Leao2 Oliveira, Joyce Moura2 Almeida, Lucas Frederico de2 Braz, Marcos2 Machado, Lídia Maria Medeiros1 Machado, Paulo Roberto Lima1 Castellucci, Lea Cristina1 |
| Institucions: | 1Instituto Nacional de Ciencia e Tecnologia em Doencas Tropicais, Salvador, Bahia. Brasil 2Universidade Federal da Bahia, Hospital Universitario Professor Edgard Santos, Salvador, Bahia. Brasil |
| Any: | 2017 |
| Període: | Abr |
| Volum: | 112 |
| Número: | 4 |
| Paginació: | 260-268 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Leprosy or hansen’s disease is a spectral disease whose clinical forms mostly depends on host’s immune and genetic factors. Different Toll-like receptors (TLR) variants have been described associated with leprosy, but with some lack of replication across different populations. OBJECTIVES To evaluate the role of polymorphisms in genes TLR1, TLR2 and TLR4 and susceptibility to leprosy in a genetic case control study; to verify the association between genotypes of these markers and the immunological profile in the serum of patients with leprosy. METHODS Pre-designed TaqMan® assays were used to genotype markers at TLR1 (rs4833095, rs5743551), TLR2 (rs7656411, rs3804099) and TLR4 (rs1927914, rs1927911). A panel of cytokines and chemokines was accessed by enzime-linked immunosorbent assay (ELISA) test in the serum of a subgroup of patients with and without leprosy reactions. FINDINGS Our results show an association between the T allele of rs3804099 at the TLR2 gene and increased risk for leprosy per se [Odds ratio (OR) = 1.296, p = 0,022]. In addition, evaluating the association between different genotypes of the TLR1, 2 and 4 markers and cytokine/chemokine serological levels, IL-17 appears as an immunological marker regulated by the polymorphism of the three TLR genes evaluated, whereas different TLR1 genotypes were associated with differential production of IL-12p40 and MCP-1(CCL2). Furthermore, other relevant serum markers such as CXCL-10 and IL-6 seemed to be regulated by TLR2 variants and IL-1β was related to TLR4 genotypes. MAIN CONCLUSIONS All together our data points that the tested TLR markers may have a regulatory role in the immunity against Mycobacterium leprae, by driving the host’s production of key cytokines and chemokines involved in the pathogenesis of this disease |
| Disciplines | Medicina |
| Paraules clau: | Dermatología, Microbiología, Genética, Lepra, Polimorfismo genético, Susceptibilidad a infecciones, Perfil inmunológico, Citocinas, Quimocinas |
| Keyword: | Medicine, Dermatology, Microbiology, Genetics, Leprosy, Genetic polymorphism, Infections susceptibility, Immune profile, Cytokines, Chemokines |
| Text complet: | Texto completo (Ver HTML) |
