| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000446396 |
| ISSN: | 0074-0276 |
| Autors: | Lima, Jhenifer Yonara de1 Santos, Marlon Dias Mariano1 Murakami, Mario Tyago2 Carvalho, Paulo Costa1 Souza, Tatiana de Arruda Campos Brasil de1 |
| Institucions: | 1Fundacao Oswaldo Cruz, Instituto Carlos Chagas, Curitiba, Parana. Brasil 2Centro Nacional de Pesquisa em Energia e Materiais, Laboratorio Nacional de Biorrenovaveis, Campinas, Sao Paulo. Brasil |
| Any: | 2021 |
| Volum: | 116 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Leishmaniasis is a neglected tropical disease caused by the parasite Leishmania braziliensis, commonly found in Brazil and associated with cutaneous and visceral forms of this disease. Like other organisms, L. braziliensis has an enzyme called glutamine synthetase (LbGS) that acts on the synthesis of glutamine from glutamate. This enzyme plays an essential role in the metabolism of these parasites and can be a potential therapeutic target for treating this disease. Investigate LbGS structure and generate structural models of the protein. We use the method of crosslinking mass spectrometry (XLMS) and generate structural models in silico using I-TASSER. 42 XLs peptides were identified, of which 37 are explained in a monomeric model with the other five indicating LbGS dimerization and pentamers interaction region. The comparison of 3D models generated in the presence and absence of XLMS restrictions probed the benefits of modeling with XLMS highlighting the inappropriate folding due to the absence of spatial restrictions. In conclusion, we disclose the conservation of the active site and interface regions, but also unique features of LbGS showing the potential of XLMS to probe structural information and explore new drugs |
| Disciplines | Biología |
| Paraules clau: | Protozoarios, Bioquímica, Proteómica estructural, Proteínas, Glutamina sintetasa, Leishmania braziliensis |
| Keyword: | Protozoa, Biochemistry, Structural proteomics, Proteins, Glutamine synthetase, Leishmania braziliensis |
| Text complet: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
