Revista: | International journal of morphology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000327880 |
ISSN: | 0717-9502 |
Autors: | Romanini, M.C1 Paz, D.A2 Rodríguez, N3 Rolando, A.N1 Mugnaini, M.T1 Soñez, C.A1 Bozzo, A1 Marioli, J.M2 Pastorino, I.C1 Gauna, H.F2 |
Institucions: | 1Universidad Nacional de Río Cuarto, Facultad de Agronomía y Veterinaria, Río Cuarto, Córdoba. Argentina 2Universidad Nacional de Buenos Aires, Facultad de Ciencias Exactas, Buenos Aires. Argentina 3Universidad Nacional de Río Cuarto, Facultad de Ciencias Exactas, Río Cuarto, Córdoba. Argentina |
Any: | 2007 |
Període: | Mar |
Volum: | 25 |
Número: | 1 |
Paginació: | 85-94 |
País: | Chile |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Descriptivo |
Resumen en inglés | The chronic stress induces functional adaptations in the hypothalamo-pituitary- adrenocortical (HPA) and in the sympathetic-medullary-adrenal axis (SAM). Both axis are considered vital regulators of the homeostasis in vertebrates (Seyle, 1936; Ostrandrer et al, 2006. On the other hand, the placenta provides highly specialized functions during gestation that are critical for the normal development of the embryo/fetus (Soares et al., 1991). We hypothesized that the chronic immobilization (IMO) stress in pregnancy rats produces alterations in prolactin concentrations in placental tissue and also changes in the response of SAM axis. Chronic stress by IMO was applied on days 12, 17 and 21 of pregnancy rats. Relative concentrations and localization of placental lactogen-II (PL-II) and the PRL- like protein A (PLP-A) in chorioalantoic placenta were estimated by Immunoblotting and Immunocytochemical analysis. The levels of catecholamines metabolite, acid 3-metoxi 4-hidroximandélico (VMA), were analyzed in stressed rats urines on 6,12,17,21 days of pregnancy, by HPLC, in order to determine the response of SAM axis. During the days of the pregnancy studied, chronic stress did not induce any changes neither in the localization nor in placental concentrations of PL-II and PLP-A. The VMA values in stressed mothers urines increased on the day 6 respecting the control ones at the same time of pregnancy. VMA values in stressed rats at 21 days of pregnancy are smaller than the respective controls. We conclude that the chronic stressed mothers activated the SAM axis at the beginning of pregnancy and then they diminished the metabolites catecholamines that were interpreted as a stress adaptation coincident with normal concentrations of both placentary prolactines at this stage of the pregnancy |
Disciplines | Medicina |
Paraules clau: | Medicina experimental, Lactogenesis, Placenta, Proteínas, Prolactina, Ratas, Estrés crónico, Animales de laboratorio |
Keyword: | Medicine, Experimental medicine, Lactogenesis, Placenta, Proteins, Prolactin, Rats, Chronic stress, Laboratory animals |
Text complet: | Texto completo (Ver HTML) |