| Revista: | International braz j urol |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000296490 |
| ISSN: | 1677-5538 |
| Autors: | Esteves, Sandro C1 Spaine, Deborah M Cedenho, Agnaldo P Srougi, Miguel |
| Institucions: | 1Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Ribeirao Preto, Sao Paulo. Brasil |
| Any: | 2003 |
| Període: | Mar-Abr |
| Volum: | 29 |
| Número: | 2 |
| Paginació: | 133-140 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Caso clínico, analítico |
| Resumen en inglés | OBJECTIVE: Comparing in human semen samples with low initial quality, the effects of 2 techniques of cryopreservation and dilution/centrifugation after thawing on the spermatic motility and vitality. MATERIALS AND METHODS: Semen samples from 15 oligo and/or asthenozoospermic individuals assisted in the infertility sector of a tertiary hospital were obtained through masturbation. The samples were divided into 2 portions of equal volume, and diluted (1:1; v/v) with the cryoprotector containing glycerol (Test yolk buffer). One portion was frozen through the technique of liquid nitrogen vapor with static phases (group I - GI), while the other was frozen through a programmable biological freezer with linear speed (Planer, Kryo 10, series III) (group II - GII). The following parameters were assessed before freezing and after thawing: percentage of spermatozoa with progressive motility (Prog%) and percentage of live spermatozoa (Vit%). After defrosting, Prog% was assessed before and after removal of cryoprotector diluent, in different time intervals (zero, 3 h, and 24 h). The statistical analysis has been accomplished by using the non-parametric tests of Wilcoxon and Friedman. RESULTS: There was significant reduction of Prog% and Vit% from before freezing to after defrosting in both groups, I and II (p < 0.001). Values of Prog% and Vit% were not statistically different between groups, after thawing. It has been observed a significant reduction in Prog% among portions frozen with the automated technique after dilution and centrifugation |
| Disciplines | Medicina |
| Paraules clau: | Cirugía, Endocrinología, Espermatozoides, Criopreservación, Motilidad, Esperma |
| Keyword: | Medicine, Endocrinology, Surgery, Spermatozoa, Cryopreservation, Motility, Sperm |
| Text complet: | Texto completo (Ver HTML) |
