Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
Título del documento: Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
Revista: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000363257
ISSN: 1415-4757
Autores: 1
2
3
2
4
5
6
2
Instituciones: 1Kaohsiung Armed Forces General Hospital, Kaohsiung. Taiwán
2National Defense Medical Center, School of Public Health, Taipei. Taiwán
3National Defense Medical Center, Institute of Life Sciences, Taipei. Taiwán
4Fu-Jen Catholic University, College of Medicine, New Taipei City. Taiwán
5Meiho University, Department of Health Business Administration, Pingtung. Taiwán
6Tri-Service General Hospital, Department of Surgery, Taipei. Taiwán
Año:
Periodo: Sep
Volumen: 36
Número: 3
Paginación: 323-328
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats. The earliest change in p16 methylation status in the DMH-treated group stool samples occurred during week nine; repeatabilities were 57.1% in week 19 (p = 0.070) and 85.7% in week 34 (p = 0.005). A temporal correlation was evidenced between progression of CRC and p16 methylation status, as evidenced by DMH-induced rat feces. Using fecal DNA methylation status to determine colorectal tissue methylation status can reveal CRC progression. Our data suggests that p16 promoter methylation is a feasible epigenetic marker for the detection and may be useful for CRC screening
Disciplinas: Medicina
Palabras clave: Diagnóstico,
Oncología,
Parasitología,
Cáncer colorrectal,
Metilación del ADN,
Examen coproparasitoscópico
Keyword: Medicine,
Diagnosis,
Oncology,
Parasitology,
Colorectal cancer,
DNA methylation,
Stool test
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