| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000408497 |
| ISSN: | 1415-4757 |
| Autors: | Conforte, Alessandra Jordano1 Guimaraes-Dias, Fabia1 Neves-Borges, Anna Cristina2 Bencke-Malato, Marta1 Felix-Whipps, Durvalina1 Alves-Ferreira, Márcio1 |
| Institucions: | 1Universidade Federal do Rio de Janeiro, Instituto de Biologia, Rio de Janeiro. Brasil 2Universidade Federal do Estado do Rio de Janeiro, Departamento de Botanica, Rio de Janeiro. Brasil |
| Any: | 2017 |
| Volum: | 40 |
| Paginació: | 226-237 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the best alternative to the generation of drought-tolerant crops. We characterized the promoter of α-galactosidase soybean (GlymaGAL) gene that was previously identified as highly up-regulated by drought stress. The β-glucuronidase (GUS) activity of Arabidopsis transgenic plants bearing 1000- and 2000-bp fragments of the GlymaGAL promoter fused to the uidA gene was evaluated under air-dried, polyethylene glycol (PEG) and salt stress treatments. After 24 h of air-dried and PEG treatments, the pGAL-2kb led to an increase in GUS expression in leaf and root samples when compared to the control samples. These results were corroborated by qPCR expression analysis of the uidA gene. The pGAL-1kb showed no difference in GUS activity between control and treated samples. The pGAL-2kb promoter was evaluated in transgenic soybean roots, leading to an increase in EGFP expression under air-dried treatment. Our data indicates that pGAL-2kb could be a useful tool in developing drought-tolerant cultivars by driving gene expression |
| Disciplines | Biología |
| Paraules clau: | Fisiología vegetal, Genética, Leguminosas, Soya, Tolerancia a la sequía, Estrés salino, Regulación génica, Alfa-galactosidasa |
| Keyword: | Biology, Genetics, Plant physiology, Legumes, Soybean, Drought tolerance, Saline stress, Gene regulation, Alpha-galactosidase |
| Text complet: | Texto completo (Ver HTML) |
