| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459138 |
| ISSN: | 1415-4757 |
| Autors: | Bach, Evelise1 Volpiano, Camila Gazolla1 Sant’Anna, Fernando Hayashi2 Passaglia, Luciane Maria Pereira1 |
| Institucions: | 1Universidade de Sao Paulo, Instituto de Biociencias, Porto Alegre, Rio Grande do Sul. Brasil 2Hospital Moinhos de Vento, Porto Alegre, Rio Grande do Sul. Brasil |
| Any: | 2023 |
| Volum: | 46 |
| Número: | 3 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | The taxonomy of Burkholderia sensu lato (s.l.) has been revisited using genome-based tools, which have helped differentiate closely related species. Many species from this group are indistinguishable through phenotypic traits and 16S rRNA gene sequence analysis. Furthermore, they also exhibit whole-genome Average Nucleotide Identity (ANI) values in the twilight zone for species circumscription (95-96%), which may impair their correct classification. In this work, we provided an updated Burkholderia s.l. taxonomy focusing on closely related species and give other recommendations for those developing genome-based taxonomy studies. We showed that a combination of ANI and digital DNA-DNA hybridization (dDDH) applying the universal cutoff values of 95% and 70%, respectively, successfully discriminates Burkholderia s.l. species. Using genome metrics with this pragmatic criterion, we demonstrated that i) Paraburkholderia insulsa should be considered a later heterotypic synonym of Paraburkholderia fungorum; ii) Paraburkholderia steynii differs from P. terrae by harboring symbiotic genes; iii) some Paraburkholderia are indeed different species based on dDDH values, albeit sharing ANI values close to 95%; iv) some Burkholderia s.l. indeed represent new species from the genomic viewpoint; iv) some genome sequences should be evaluated with care due to quality concerns |
| Disciplines | Biología |
| Paraules clau: | Bacterias, Taxonomía y sistemática, Burkholderia sensu, Genoma, Paraburkholderia, ADN, Hibridización |
| Keyword: | Bacteria, Taxonomy and systematics, Burkholderia sensu, Genome, Paraburkholderia, DNA, Hybridization |
| Text complet: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
