| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000418599 |
| ISSN: | 1415-4757 |
| Autors: | Pinheiro, Danilo do Rosario1 Harada, Maria Lucia1 Burbano, Rommel Mario Rodríguez2 Borges, Barbara do Nascimento1 |
| Institucions: | 1Universidade Federal do Para, Instituto de Ciencias Biologicas, Belem, Para. Brasil 2Universidade Federal do Para, Human Cytogenetics Laboratory, Belem, Para. Brasil |
| Any: | 2018 |
| Període: | Jun |
| Volum: | 41 |
| Número: | 2 |
| Paginació: | 496-501 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico |
| Resumen en inglés | Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry (IHQ). A total of 20 sam- ples were obtained. A 675 bp fragment of the S. apella COX-2 promoter region was obtained, and it was 99.2% and 68.2% similar to H. sapiens and S. boliviensis, respectively. Similar to humans, several transcription factors and cis-regulatory element binding sites were identified in the S. apella sequence. MSP revealed that all samples were methylated. However, IHQ results demonstrated positive COX-2 expression in all pre-neoplastic and tumoral sam- ples. The results suggest that the analyzed fragment is not crucial in COX-2 regulation of GC in S. apella |
| Disciplines | Medicina |
| Paraules clau: | Gastroenterología, Oncología, Mamíferos, PTGS2, Regulación génica, Modelos animales, Sapajus apella, Cáncer gástrico |
| Keyword: | Gastroenterology, Oncology, Mammals, PTGS2, Genetic regulation, Animal models, Sapajus apella, Gastric cancer |
| Text complet: | Texto completo (Ver PDF) |
