| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000359876 |
| ISSN: | 1415-4757 |
| Autores: | Rossi, Ciro Cesar1 Araujo, Elza Fernandes de1 Queiroz, Marisa Vieira de1 Bazzolli, Denise Mara Soares1 |
| Instituciones: | 1Universidade Federal de Vicosa, Departamento de Microbiologia, Vicosa, Minas Gerais. Brasil |
| Año: | 2013 |
| Periodo: | Jun |
| Volumen: | 36 |
| Número: | 2 |
| Paginación: | 243-251 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The OmlA protein is a virulence factor of Actinobacillus pleuropneumoniae, an important pathogen in pigs. The polymorphisms present in the omlA gene sequence of 15 reference serotypes of A. pleuropneumoniae and non-serotypable isolates were assessed to determine the possible evolutionary relationship among them and to validate the importance of this gene as a molecular marker for the characterization of this bacterium. Divergence among the 15 serotypes of A. pleuropneumoniae probably resulted initially from two major evolutionary events that led to subsequent differentiation into nine groups. This differentiation makes it possible to characterize most of the serotypes by using bionformatics, thereby avoiding problems with immunological cross-reactivity. A conserved α-helix common to all the serotypes was most likely involved in connecting the protein to the outer membrane and acting as a signal peptide. A previously unknown gene duplication was also identified and could contribute to the genetic variability that makes it difficult to serotype some isolates. Our data support the importance of the omlA gene in the biology of A. pleuropneumoniae and provide a new area of research into the OmlA protein |
| Disciplinas: | Biología |
| Palabras clave: | Bacterias, Genética, Factores de virulencia, Caracterización genética, Análisis filogenético, Pleuroneumonía porcina, Actinobacillus pleuropneumoniae |
| Keyword: | Biology, Bacteria, Genetics, Virulence factors, Genetic characterization, Phylogenetic analysis, Porcine pleuropneumonia, Actinobacillus pleuropneumoniae |
| Texto completo: | Texto completo (Ver HTML) |
