| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000331372 |
| ISSN: | 1415-4757 |
| Autors: | Marques, Abi S.A1 Marchaison, Anne2 Gardan, Louis2 Samson, Regine2 |
| Institucions: | 1Empresa Brasileira de Pesquisa Agropecuaria, Recursos Geneticos e Biotecnologia, Brasilia, Distrito Federal. Brasil 2Institut National de la Recherche Agronomique, Station de Pathologie Vegetale, Angers, Maine-et-Loire. Francia |
| Any: | 2008 |
| Període: | Mar |
| Volum: | 31 |
| Número: | 1 |
| Paginació: | 106-115 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | The phenotypic characteristics and genetic fingerprints of a collection of 120 bacterial strains, belonging to Pseudomonas syringae sensu lato group, P. viridiflava and reference bacteria were evaluated, with the aim of species identification. The numerical analysis of 119 nutritional characteristics did not show patterns that would help with identification. Regarding the genetic fingerprinting, the results of the present study supported the observation that BOX-PCR seems to be able to identify bacterial strains at species level. After numerical analyses of the bar-codes, all pathovars belonging to each one of the nine described genomospecies were clustered together at a distance of 0.72, and could be separated at genomic species level. Two P. syringae strains of unknown pathovars (CFBP 3650 and CFBP 3662) and the three P. syringae pv. actinidiae strains were grouped in two extra clusters and might eventually constitute two new species. This genomic species clustering was particularly evident for genomospecies 4, which gathered P. syringae pvs. atropurpurea, coronafaciens, garçae, oryzae, porri, striafaciens, and zizaniae at a noticeably low distance |
| Disciplines | Biología, Química |
| Paraules clau: | Bacterias, Genética, Bioquímica, Pseudomonas syringae, Caracterización fenotípica, Genomoespecies, BOX-PCR, Identificación bacteriana |
| Keyword: | Biology, Chemistry, Bacteria, Genetics, Biochemistry, Pseudomonas syringae, Phenotypic characterization, Genomospecies, BOX-PCR, Bacterial identification |
| Text complet: | Texto completo (Ver HTML) |
