| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459282 |
| ISSN: | 1415-4757 |
| Autors: | Carratto, Thássia Mayra Telles1 Moraes, Vitor Matheus Soares1 Recalde, Tamara Soledad Frontanilla2 Oliveira, Maria Luiza Guimarães de2 Teixeira Mendes-Junior, Celso1 |
| Institucions: | 1Universidade de Sao Paulo, Faculdade de Filosofia, Ribeirao Preto, Sao Paulo. Brasil 2Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil |
| Any: | 2022 |
| Volum: | 45 |
| Número: | 3 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Descriptivo |
| Resumen en inglés | Massively parallel sequencing, also referred to as next-generation sequencing, has positively changed DNA analysis, allowing further advances in genetics. Its capability of dealing with low quantity/damaged samples makes it an interesting instrument for forensics. The main advantage of MPS is the possibility of analyzing simultaneously thousands of genetic markers, generating high-resolution data. Its detailed sequence information allowed the discovery of variations in core forensic short tandem repeat loci, as well as the identification of previous unknown polymorphisms. Furthermore, different types of markers can be sequenced in a single run, enabling the emergence of DIP-STRs, SNP-STR haplotypes, and microhaplotypes, which can be very useful in mixture deconvolution cases. In addition, the multiplex analysis of different single nucleotide polymorphisms can provide valuable information about identity, biogeographic ancestry, paternity, or phenotype. DNA methylation patterns, mitochondrial DNA, mRNA, and microRNA profiling can also be analyzed for different purposes, such as age inference, maternal lineage analysis, body-fluid identification, and monozygotic twin discrimination. MPS technology also empowers the study of metagenomics, which analyzes genetic material from a microbial community to obtain information about individual identification, post-mortem interval estimation, geolocation inference, and substrate analysis. This review aims to discuss the main applications of MPS in forensic genetics |
| Disciplines | Biología, Medicina |
| Paraules clau: | Genética, Medicina social, Genética forense, ADN, RNA, Secuenciación de próxima generación (NGS), Revisión bibliográfica |
| Keyword: | Genetics, Social medicine, Forensic genetics, Bibliographic review, Next Generation Sequencing (NGS), DNA, RNA |
| Text complet: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
