| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358441 |
| ISSN: | 0717-3458 |
| Autors: | González, Germán Andrés1 Pacheco, María Gabriela1 Oneto, Cecilia Décima1 Etchart, Valeria J1 Kandus, Mariana V1 Salerno, Juan Carlos1 Eyherabide, Guillermo2 Presello, Daniel2 Lewi, Dalia Marcela1 |
| Institucions: | 1Instituto de Genética Ewald A. Favret, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Castelar, Buenos Aires. Argentina 2Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Pergamino, Pergamino, Buenos Aires. Argentina |
| Any: | 2012 |
| Volum: | 15 |
| Número: | 1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC (mature somatic embryos/callus evaluated) x 100], Regeneration Capacity (RC) and Fertile Plant Recovery in greenhouse (FPR, fertile plants/regenerated plants) were recorded. A total of 17 lines reached an EC > 50%, and 14 out of those 17 lines regenerated seedlings. The FPR ranged between 50 and 100%. Also, we selected three promising lines with high agronomic performance, as alternatives to Hi II, in order to be included in a maize transformation scheme via somatic embryogenesis. In addition, we report the usefulness of Single Sequences Repeat (SSRs) in the determination of genetic diversity among 14 divergent lines for somatic embryogenesis response. The seven lines displaying good in vitro behaviour can be crossed to obtain hybrids combining desirable alleles for somatic embryogenesis response and different genetic backgrounds |
| Disciplines | Agrociencias |
| Paraules clau: | Fitotecnia, Gramíneas, Fisiología vegetal, Líneas endogámicas, Embriogénesis somática, Marcadores moleculares, Maíz, Regeneración vegetal, Zea mays |
| Keyword: | Agricultural sciences, Crop husbandry, Gramineae, Plant physiology, Inbred lines, Somatic embryogenesis, Molecular markers, Corn, Plant regeneration, Zea mays |
| Text complet: | Texto completo (Ver HTML) |
