Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut



Título del documento: Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
Revista: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000336123
ISSN: 0717-3458
Autors: 1
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Institucions: 1Shandong Peanut Research Institute, Qingdao. China
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Període: Sep
Volum: 13
Número: 5
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut
Disciplines Biología,
Agrociencias
Paraules clau: Genética,
Fitotecnia,
Biotecnología,
Cacahuate,
Mejoramiento genético,
Identificación molecular,
Híbridos,
PCR
Keyword: Biology,
Agricultural sciences,
Genetics,
Crop husbandry,
Biotechnology,
Peanut,
Plant breeding,
Molecular identification,
Hybrids,
PCR
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