| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000359577 |
| ISSN: | 0717-3458 |
| Autors: | Sattayasamitsathit, Supalak1 Methacanon, Pawadee2 Prasertsan, Poonsuk3 |
| Institucions: | 1Prince of Songkla University, Faculty of Agro-Industry, Hatyai. Tailandia 2National Metal and Materials Technology Center, Pathum Thani. Tailandia 3Prince of Songkla University, Faculty of Agro-Industry, Hatyai, Songkhla. Tailandia |
| Any: | 2011 |
| Volum: | 14 |
| Número: | 6 |
| Paginació: | 4-4 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | The batch fermentation of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae SU6 at different crude glycerol concentration (40-100 g l-1), pH (6.5-7.5) and temperature (31-40ºC) combined with two-phase pH-controlled strategy was investigated. Effect of feeding rate (0.10-0.15 L h-1) was studied in fed-batch fermentation. In batch fermentation, the optimal condition was 60 g l-1 crude glycerol, pH control at 6.5 and cultivation temperature at 37ºC. The maximum 1,3-PD of 20 g l-1, the yield of 0.34 g 1,3-PD g-1 glycerol consumed and the productivity of 1.25 g l-1 h-1 were achieved at 16 hrs cultivation. The by-products were acetic acid and succinic acid at 2.7 and 1.1 g l-1, respectively. Two-phase pH-controlled strategy gave better results (24.95 g l-1 1,3-PD and 1.78 g l-1 h-1 productivity) than constant pH-controlled strategy (20 g l-1 and 1.25 g l-1 h-1, respectively) at 16 hrs incubation. In fed-batch fermentation, the maximum 1,3-PD of 45.35 g l-1 was achieved at constant feeding rate of 0.1 L h-1. The yield and productivity were 0.44 g g-1 and 1.94 g l-1 h-1, respectively. The fed-batch fermentation with constant feeding at 0.1 L h-1 with two-phase pH-controlled strategy gave 2.2 folds higher 1,3 PD concentration than the batch fermentation with two-phase pH-controlled strategy. This demonstrated the great impact of combination of pH control and feeding strategies in fed-batch fermentation on enhancing 1,3-propanediol production |
| Disciplines | Ingeniería, Química, Biología |
| Paraules clau: | Ingeniería química, Fermentaciones, Bacterias, 1,3-propanodiol, Fermentación, Klebsiella pneumoniae, Glicerol, Biotecnología |
| Keyword: | Engineering, Chemistry, Biology, Chemical engineering, Fermentation, Bacteria, 1,3-propanediol, Fermentation, Klebsiella pneumoniae, Glycerol, Biotechnology |
| Text complet: | Texto completo (Ver PDF) |
