| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000336137 |
| ISSN: | 0717-3458 |
| Autors: | Yuan, Mei1 Gong, Limin2 Meng, Ronghua3 Li, Shuangling1 Dang, Phat4 Guo, Baozhu5 He, Guohao2 |
| Institucions: | 1Shandong Peanut Research Institute, Qingdao. China 2Tuskegee University, College of Agricultural, Environmental and Natural Sciences, Tuskegee, Alabama. Estados Unidos de América 3University of Pennsylvania, School of Medicine, Filadelfia, Pensilvania. Estados Unidos de América 4United States Department of Agriculture, National Peanut Research Laboratory, Dawson, Georgia. Estados Unidos de América 5United States Department of Agriculture, Crop Genetics and Breeding Research Unit, Tifton, Georgia. Estados Unidos de América |
| Any: | 2010 |
| Període: | Nov |
| Volum: | 13 |
| Número: | 6 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was observed in cultivated peanut by these new developed SSRs, a high level of transferability to wild species would be beneficial to increasing the number of SSRs in wild species |
| Disciplines | Biología |
| Paraules clau: | Angiospermas, Genética, Cacahuate, Marcadores genéticos, Microsatélites, Polimorfismo genético, Arachis hypogaea |
| Keyword: | Biology, Angiosperms, Genetics, Peanut, Genetic markers, Microsatellites, Genetic polymorphism, Arachis hypogaea |
| Text complet: | Texto completo (Ver PDF) |
