| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000336246 |
| ISSN: | 0717-3458 |
| Autors: | Rustiguel, Cynthia Barbosa1 Oliveira, Arthur Henrique Cavalcanti de1 Terenzi, Hector Francisco1 Jorge, Joao Atilio1 Guimaraes, Luis Henrique Souza1 |
| Institucions: | 1Universidade de Sao Paulo, Faculdade de Filosofia, Ciencias e Letras, Ribeirao Preto, Sao Paulo. Brasil |
| Any: | 2011 |
| Període: | Mar |
| Volum: | 14 |
| Número: | 2 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The filamentous fungus A. phoenicis produced high levels of β-D-fructofuranosidase (FFase) when grown for 72 hrs under Solid-State Fermentation (SSF), using soy bran moistened with tap water (1:0.5 w/v) as substrate/carbon source. Two isoforms (I and II) were obtained, and FFase II was purified 18-fold to apparent homogeneity with 14% recovery. The native molecular mass of the glycoprotein (12% of carbohydrate content) was 158.5 kDa with two subunits of 85 kDa estimated by SDS-PAGE. Optima of temperature and pH were 55ºC and 4.5. The enzyme was stable for more than 1 hr at 50ºC and was also stable in a pH range from 7.0 to 8.0. FFase II retained 80% of activity after storage at 4ºC by 200 hrs. Dichroism analysis showed the presence of random and β-sheet structure. A. phoenicis FFase II was activated by Mn2+, Mg2+ and Co2+, and inhibited by Cu2+, Hg2+ and EDTA. The enzyme hydrolyzed sucrose, inulin and raffinose. Kd and Vmax values were 18 mM and 189 U/mg protein using sucrose as substrate |
| Disciplines | Biología |
| Paraules clau: | Biotecnología, Fermentación en sustrato sólido, Sustrato, Soya, Aspergillus phoenicis, Fructofuranosidasa, Sacarosa |
| Keyword: | Biology, Biotechnology, Solid state fermentation, Substrates, Soybean, Aspergillus phoenicis, Fructofuranosidase, Sucrose |
| Text complet: | Texto completo (Ver PDF) |
