| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358466 |
| ISSN: | 0717-3458 |
| Autors: | Ding-ping, Bai1 Cheng-jiang, Yu1 Yu-lin, Chen1 |
| Institucions: | 1Northwest A & F University, College of Animal Science and Technology, Yangling, Shaanxi. China |
| Any: | 2012 |
| Volum: | 15 |
| Número: | 2 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reproduction breeds and two seasonal reproduction breeds. One mutation at exon 3 (NM_001009461:c.486A > G) was firstly described at the sheep AA-NAT locus. Hence, we described the SmaI PCR-RFLP method for detecting EX3 486A > G mutation, frequencies of the AA-NAT-G allele varied from 0.871 to 0.908 in two non-seasonal reproduction breeds and 0.517 to 0.578 in two seasonal reproduction breeds. The associations of SmaI polymorphism with estrus traits was analyzed in non-seasonal reproduction breeds sheep and seasonal reproduction breeds sheep, the significant statistical results were found between them, the GG genotype frequencies was higher in non-seasonal reproduction breeds (p < 0.001), while, the GA genotype frequencies was higher in seasonal reproduction breeds (p < 0.05). Hence, the EX3 486A > G mutation could facilitate association analysis and serve as a genetic marker for Chinese sheep breeding and genetics |
| Disciplines | Medicina veterinaria y zootecnia |
| Paraules clau: | Ovinos y caprinos, Genética, Mejoramiento genético, Polimorfismo genético, Melatonina, Desempeño reproductivo, Ovejas |
| Keyword: | Veterinary medicine and animal husbandry, Sheep and goats, Genetics, Genetic improvement, Genetic polymorphism, Reproductive performance, Melatonin, Sheep |
| Text complet: | Texto completo (Ver HTML) |
