| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451459 |
| ISSN: | 1984-8250 |
| Autors: | Araujo, Elaine de Oliveira1 Rosa, Aline Marques2 Amaral, Marcos Serrou do3 Sversut, Rúbia Adrieli2 Baroni, Adriano César de Morais2 Oliveira, Lincoln Carlos Silva de4 Kassab, Nájla Mohamad2 |
| Institucions: | 1Universidade Federal do Mato Grosso do Sul, Hospital Universitario Maria Aparecida Pedrossian, Campo Grande, Mato Grosso do Sul. Brasil 2Universidade Federal do Mato Grosso do Sul, Faculdade de Ciencias Farmaceuticas, Campo Grande, Mato Grosso do Sul. Brasil 3Universidade Federal do Mato Grosso do Sul, Instituto de Fisica, Campo Grande, Mato Grosso do Sul. Brasil 4Universidade Federal do Mato Grosso do Sul, Instituto de Quimica, Campo Grande, Mato Grosso do Sul. Brasil |
| Any: | 2020 |
| Volum: | 56 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | We developed and validated a stability-indicating assay method for the simultaneous determination of enrofloxacin and piroxicam in combination and in the presence of degradation products. Reverse-phase high-performance liquid chromatography analyses were carried out on a Vertisep C18 column and acetonitrile-water (48:52 v/v, pH 3.0) mobile phase with a 1.00 mL min−1 flow rate. The efficient chromatographic separation of these drugs and their forced degradation products was achieved in less than 5min with a peak purity match factor higher than 950. The method used showed linearity in the concentration ranges of 0.25 to 16.0 µg mL−1 for enrofloxacin (r = 0.9997) and 0.125 to 8.0 µg mL−1 for piroxicam (r = 0.9999) as well as precision (relative standard deviation lower than 2%), accuracy (mean recovery 100 ± 2%), and robustness, according to ICH (International Conference on Harmonization) and AOAC (Association of Official Analytical Chemists) guidelines. This method can simultaneously determine the combination of these drugs in a veterinary formulation and separate the drug peaks from their forced degradation products. Additionally, its optimized chromatographic conditions can contribute to the quality control of this formulation in pharmaceutical manufacturing plants and minimize waste from the organic solvent |
| Disciplines | Química |
| Paraules clau: | Química farmacéutica, Química analítica, Antiinflamatorios, Formulaciones farmacéuticas, Enrofloxacina, Piroxicam, Fluoroquinolonas, Cromatografía líquida de alta resolución (HPLC), Estabilidad química |
| Keyword: | Medicinal chemistry, Analytical chemistry, Antiinflammatory agents, Pharmaceutical formulations, Enrofloxacin, Piroxicam, Fluoroquinolones, High performance liquid chromatography (HPLC), Chemical stability |
| Text complet: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
