| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000450967 |
| ISSN: | 1984-8250 |
| Autors: | Sher, Nawab1 Fatima, Nasreen1 Perveen, Shahnaz2 Siddiqui, Farhan Ahmed3 |
| Institucions: | 1University of Karachi, Faculty of Science, Karachi, Baluchistán. Pakistán 2Pakistan Council of Scientific and Industrial Research Laboratories Complex, Karachi, Baluchistán. Pakistán 3Federal Urdu University Arts, Science and Technology, Faculty of Pharmacy, Karachi, Baluchistán. Pakistán |
| Any: | 2019 |
| Volum: | 55 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | A novel reverse phase, isocratic HPLC method is described to separate five anti-diabetic drugs i.e., glimepiride, metformin, sitagliptin, rosiglitazone and pioglitazone. Nucleosil C18 analytical column was used as stationary phase, while mobile phase consisted of acetonitrile:phosphate buffer: methanol (40/40/20, v/v) pH 2.0. Effluent was monitored at a flow rate 1 mL/min and detected at wavelength of 240 nm. This research produced excellent chromatography over a wide concentration range of 25-10000 ng/mL. Sepprated and well resolved quantifiable peaks were obtained and test results were linear in this range. Correlation coefficient of more than 0.9990 was witnessed as well as Low %RSD values i.e., maximum 2.0% documented excellent precision of the method. Good recoveries from pharmaecutical (99-101%), urine and plasma samples (>96%) in a range of concentrtion granted very good linearity, accuracy and precision. The projected method has satisfactory applications in quality control of these molecules as well as quantification of these molecules in urine and plasma samples |
| Disciplines | Química |
| Paraules clau: | Química farmacéutica, Química analítica, Metformina, Liberación de fármacos, Diabetes mellitus, Farmacocinética, Cromatografía líquida de alta resolución (HPLC) |
| Keyword: | Medicinal chemistry, Analytical chemistry, Metformin, Drug delivery, Pharmacokinetics, High performance liquid chromatography (HPLC), Diabetes mellitus |
| Text complet: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
