| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451481 |
| ISSN: | 1984-8250 |
| Autors: | Silva, Isabel Cristina Vieira da1 Oliveira, Pollyana Felix de1 Barbosa, Gleyce Moreno1 Wessjohann, Ludger A2 Cardozo-Filho, Lucio3 Holandino, Carla1 Muzitano, Michelle Frazão1 Leal, Ivana Correa Ramos1 |
| Institucions: | 1Universidade Federal do Rio de Janeiro, Faculdade de Farmacia, Rio de Janeiro. Brasil 2Leibniz Institute of Plant Biochemistry, Bioorganic Chemistry Department,, Halle, Saale. Alemania 3Centro Universitario Fundacao de Ensino Octavio Bastos, Sao Joao da Boa Vista, Sao Paulo. Brasil |
| Any: | 2020 |
| Volum: | 56 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Cancer is one of the most prevalent diseases worldwide and the natural products could be a source of bioactive compounds. Passiflora mucronata (PM) belongs to a very known vegetal genus, although, there are no studies about cytotoxic activity or isolated compounds. Different extracts from PM were obtained by liquid-liquid partition (P), Soxhlet (Sox) and supercritical fluid (SFE1-5) extraction techniques, being compared concerning their yields, chemical profile and cytotoxicity. The Sox extracts showed the highest yields (6.03%: hexane; 2.51%: dichloromethane) followed by SFE (from 4.34 to 1.63%) and partitions (1.06 and 2.26%). The hexane partition (HP) showed the best cytotoxic activity against K562 cell line (IC50 = 18.72 µg.mL-1). From HP, the following compounds were identified and analysed its cytotoxic activities: β-amyrin (IC50 = 3.92 µg.mL-1), β-sitosterol (IC50 = 3.37 µg.mL-1), stigmasterol (IC50 = 3.31 µg.mL-1) and oleanolic acid. Stigmasterol induced about 75% of K562 total apoptosis. The compounds were tested against MA-104 cell line and the selective index (SI) attributed (SI >10 for all compounds). This indicates good selectivity to K562 cell line at the expense of MA-104. This is the first time, identifying those compounds to PM |
| Disciplines | Biología, Química |
| Paraules clau: | Fitoquímica, Farmacología, Extractos vegetales, Estigmasterol, Triterpenos, Citotoxicidad, Apoptosis, Passiflora mucronata, Passifloraceae |
| Keyword: | Phytochemistry, Pharmacology, Plant extracts, Stigmasterol, Triterpenes, Cytotoxicity, Apoptosis, Passiflora mucronata, Passifloraceae |
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