Late cardiac perconditioning by phenylephrine in an isolated rat heart model is mediated by mitochondrial potassium channels
Título del documento: Late cardiac perconditioning by phenylephrine in an isolated rat heart model is mediated by mitochondrial potassium channels
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451011
ISSN: 1984-8250
Autors: 1
2
1
3
4
2
Institucions: 1Lorestan University of Medical Sciences, Department of Physiology, Khorramabad. Irán
2Lorestan University of Medical Sciences, Student Research Committee, Khorramabad. Irán
3Iran University of Medical Sciences, School of Medicine, Teherán. Irán
4Gonabad University of Medical Sciences, School of Medicine, Gonabad, Razavi Khorasan. Irán
Any:
Volum: 55
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés The present study was designed to investigate the effect of early and late administration of phenylephrine during ischemia against regional ischemia-reperfusion injuries in an isolated rat heart model. All animals were randomly divided into experimental groups: (I) IR (Ischemic/ reperfusion): the hearts underwent 35 min of regional ischemia followed by 60 min of reperfusion; (II) 5HD-IR-0: the hearts were perfused for 5 min with 5HD (5-hydroxydecanoate, specific mKATP channel blocker, 100 µM) at the onset of regional ischemia; (III) 5HD-IR-20: the hearts were perfused for 5 min with 5HD 20 min after regional ischemia; (IV) PE-IR-10: the hearts were perfused for 5 min with phenylephrine 10 min after regional ischemia; (V) PE-IR-30: the hearts were perfused for 5 min with phenylephrine (100 µM) 30 min after regional ischemia; (VI) PE-5HD-IR-10 group: the hearts were perfused for 5 min with 5HD at the onset of regional ischemia after which phenylephrine was administrated as in group IV; and (VII) PE-5HD-IR-30: the hearts were perfused for 5 min with 5HD 20 min after the ischemia and then phenylephrine was administrated as in group V. The hemodynamic parameters were recorded throughout the experiment. Ischemia-induced arrhythmias, myocardial infarct size (IS), creatin kinase-MB isoenzyme (CK-MB), plasma lactate dehydrogenase (LDH) activities, and coronary blood flow (CBF) were measured in all animals. Perfusion of phenylephrine 30 min after the regional ischemia curtailed the myocardial infarct size, reduced CK-MB, and improved cardiac function and CBF. Administration of 5HD 30 min after the ischemia abolished cardioprotective effects of phenylephrine in the late phase. These results suggest the involvement of mKATP in the mechanism of phenylephrine-induced late preconditioning
Disciplines Medicina,
Química
Paraules clau: Sistema cardiovascular,
Farmacología,
Fisiopatología,
Isquemia,
Reperfusión,
Precondicionamiento,
Cardioprotección,
Fenilefrina,
Canales de potasio,
Mitocondria
Keyword: Cardiovascular system,
Pharmacology,
Physiopathology,
Ischemia,
Reperfusion,
Preconditioning,
Cardioprotection,
Phenylephrine,
Potassium channels,
Mitochondria
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